Functional analysis of TRPV1 channels in vestibular system

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791894
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Osaka University
• Principal Investigator: KAMAKURA Takefumi 大阪大学, 医学部附属病院, 医員 (30600564)
• Project Period (FY): 2011 – 2012
• Keywords: 前庭神経節 / TRPV1受容体 / TRPA1受容体 / カルシウムイメージング

Research Abstract

TRPV1 is expressed in sensory neurons such as dorsal root ganglia (DRG) and trigeminal ganglia (TG), and are involved in nociception, being activated by nociceptive stimuli. We investigated the expression of TRPV11 in rat VG neurons by RT-PCR, in situ hybridization, immunohistochemistry, and Ca^2+ imaging experiments, and we revealed that TRPV1 is functional as an ion channel and that co-expressed with TRPA1. In summary, our histological and physiological studies reveal that TRPV1 and TRPA1 are expressed in VG neurons. It is suggested that TRPV1 and TRPA1 in VG neurons might participate in vestibular function and/or dysfunction such as vertigo.

Research Products

• [Presentation] Functional Analysis of TRPV1 and TRPA1 in rat vestibular ganglia (2013)
  • Author(s): Kamakura T, Ishida Y, Nakamura Y, Yamada T, Kitahara T, Takimoto Y, Horii A, UnoA, Imai T, Okazaki S, Inohara H and ShimadaS
  • Organizer: 36th ARO Midwinter Meeting
  • Place of Presentation: アメリカ合衆国、ボルティモア
  • Year and Date: 20130216-17
• [Presentation] ラットに対する塩化カリウム溶液鼓室内注入による眼振誘発とその経時的変化について (2012)
  • Author(s): 鎌倉武史、北原糺、滝本泰光、宇野敦彦、今井貴夫、堀井新、岡崎鈴代、猪原秀典
  • Organizer: 第71回日本めまい平衡医学会総会・学術講演会
  • Place of Presentation: 東京
  • Year and Date: 20121128-30
• [Presentation] 前庭神経節のおける TRPV1、TRPA1 受容体の発現について (2012)
  • Author(s): 鎌倉武史、石田雄介、中村雪子、山田貴博、北原糺、滝本泰光、宇野敦彦、今井貴夫、堀井新、岡崎鈴代、猪原秀典、島田昌一
  • Organizer: 第1回耳鼻咽喉科ニューロサイエンス研究会
  • Place of Presentation: 大阪
  • Year and Date: 2012-08-25
• [Presentation] Functional expression of TRPA1 and TRPV1 in rat vestibular ganglia (2012)
  • Author(s): Kamakura T, Ishida Y, Nakamura Y, Yamada T, Kitahara T, Takimoto Y, Horii A, UnoA, Imai T, Okazaki S, Inohara H and ShimadaS
  • Organizer: 27thBarany Society Meeting
  • Place of Presentation: スウェーデン、ウプサラ
  • Year and Date: 2012-06-12