2012 Fiscal Year Final Research Report
Effect of in vivo over-expression of KGF by electroporativelytransfected KGF cDNA on the histology of middle ear cholesteatoma.
| Project/Area Number |
23791906
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Nagasaki University |
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Principal Investigator |
FUKUDA Tomomi 長崎大学, 大学院・医歯薬学総合研究科, 講師 (40372776)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 真珠腫性中耳炎 / KGF / KGFR |
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| Research Abstract |
In our previous study, we have indicated that keratinocyte growth factor (KGF) plays an important role in middle ear cholesteatoma formation. KGF is a mesenchymal-cell-derived paracrine growth factor that specifically stimulates epithelial cell growth. In this study, we investigated the effects of over-expressed KGF in vivo. As results, a single injection of KGF expression vector coupled with electroporation induced inflammatory reaction and increased proliferative activity of epithelial cells in external auditory canal, and consequently KGF may possibly induce a cholesteatoma formation. Treatment of rats of the long-term model with SU5402, a KGFreceptor inhibitor, prevented cholesteatoma formation.
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