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2012 Fiscal Year Final Research Report

Cell therapy for hereditary deafness targeting cochlear fibrocyte

Research Project

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Project/Area Number 23791937
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Otorhinolaryngology
Research InstitutionJuntendo University

Principal Investigator

KAMIYA Kazusaku  順天堂大学, 医学部, 講師 (10374159)

Project Period (FY) 2011 – 2012
Keywords耳科学
Research Abstract

In this study, we developed a novel animal model for acute sensorineural hearing loss due to fibrocyte dysfunction and performed cell therapy with bone marrow mesenchymal stem cells (MSC) as supplementation of cochlear fibrocytes functioning for cochlear ion transport. We injected MSC into the lateral semicircular canal and a number of these stem cells were then detected in the injured area in the lateral wall. The transplanted animals showed a significantly higher hearing recovery ratio than controls. We analyzed the machinery of this stem cell induction to the targeted site in cochlea and found that monocyte chemotactic protein 1 (MCP1) and chemokine (C-C motif) receptor 2 (CCR2) playedimportant roles for this cell induction. To enhance the MSC induction in cochlea, we developed a novel transplant strategy by induction of MCP1 expression in host cochlear tissue and forced expression of CCR2 in MSC. Furthermore, we developed a novel mouse model for Connexin26 mutation as most frequent heredity deafness in human. With these animal models, the developed stem cells and the novel strategy to enhance the stem cell induction, we examined to establish an efficient stem cell therapy to cochlear target site followed by recovery of hearing function in heredity deafness.

  • Research Products

    (4 results)

All 2013 2012 2011

All Journal Article (4 results) (of which Peer Reviewed: 2 results)

  • [Journal Article] KurodaInfant Calming Responses During Maternal Carrying In Humans and Mice2013

    • Author(s)
      Gianluca Esposito, Sachine Yoshida, Ryuko Ohnishi, Yousuke Tsuneoka, Maria del Carmen Rostagno, Susumu Yokota, Shota Okabe, Kazusaku Kamiya, Mikio Hoshino, Masaki Shimizu, Paola Venuti, Takefumi Kikusui, Tadafumi Kato, Kumi O
    • Journal Title

      Current Biology

      Volume: 23(9) Pages: 739-45

    • Peer Reviewed
  • [Journal Article] Cell therapy for hereditary hearing loss with stem cell homing factors2013

    • Author(s)
      Kamiya K
    • Journal Title

      Nihon Yakurigaku Zasshi

      Volume: 141(4) Pages: 191-4

  • [Journal Article] Gene transfer targeting mouse vestibule using adenovirus and adeno-associated virus vectors2012

    • Author(s)
      Hiroko Okada, Takashi Iizuka, Hideki Mochizuki, Tomoko Nihira, Kazusaku Kamiya, Ayako Inoshita, Hiromi Kasagi, Misato Kasai, Katsuhisa Ikeda
    • Journal Title

      Otology & Neurotology

      Volume: 33(4) Pages: 655-9

    • Peer Reviewed
  • [Journal Article] 多能性幹細胞を用いた遺伝性難聴に対する内耳細胞治療法の開発2011

    • Author(s)
      神谷和作 池田勝久
    • Journal Title

      Inner ear cell therapy for hereditary deafness with multipotent stem cells

      Volume: 69(12) Pages: 2215-2219

URL: 

Published: 2014-09-25  

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