Observation of limbal stem cell niche using in vivo confocal microscope

2013 Fiscal Year Final Research Report

• Project/Area Number: 23791962
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Osaka University
• Principal Investigator: OIE Yoshinori 大阪大学, 医学(系)研究科(研究院), 助教 (20599881)
• Project Period (FY): 2011 – 2013
• Keywords: 眼発生・再生医学

Research Abstract

Longest diameter and dimension of limbal basal cells were evaluated using in vivo confocal miscroscope (Heidelberg Retina Tomograph 2 Rostock Corneal Module) for patients with Harada disease. Sixteen eyes of nine patients were included. Average age was 51, 13 eyes with men, 3 eyes with women. Longest diameter and dimension of limbal basal cells in patients with Harada disease were 16.1um and 135um2, respectively. It was suggested that limbal basal cells in Harada disease were larger than in normal eyes because of destruction of melanocyte in limbal niche.

Research Products

• [Journal Article] Rigid gas permeable contact lens-assisted cataract surgery in patients with severe keratoconus (2014)
  • Author(s): Oie Y, Kamei M, Matsumura N, Fujimoto H, Soma T, Koh S, Tsujikawa M, Maeda Nishida K
  • Journal Title: J Cataract Refract Surg Volume: 40 Pages: 345-8
• [Journal Article] Development of a cell sheet transportation technique for regenerative medicine (2014)
  • Author(s): Oie Y, Nozaki T, Takayanagi H, Hara S, Hayashi R, Takeda S, Mori K, Moriya N, Soma T, Tsujikawa M, Saito K, Nishida K
  • Journal Title: Tissue Eng Part C Methods Volume: 20 Pages: 373-82
• [Journal Article] ヒト臨床試験のための培養口腔粘膜上皮細胞シートの輸送技術開発 (2014)
  • Author(s): 大家義則
  • Journal Title: 再生医療 Volume: 13 Pages: 58-63
• [Journal Article] Regenerative medicine for the cornea (2013)
  • Author(s): Oie Y, Nishida K
  • Journal Title: Biomed Res Int Volume: 2013 Pages: 428247
• [Journal Article] Effect of instillation of eyedrops for dry eye on optical quality (2013)
  • Author(s): Koh S, Maeda N, Ikeda C, Takai Y, Fujimoto H, Oie Y, Soma T, Tsujikawa M, Nishida K
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 54 Pages: 4927-33
• [Presentation] タイ王国における培養口腔粘膜上皮細胞シートを用いた角膜上皮再生治療および遠隔診療の実施 (2014)
  • Author(s): 大家義則、小林由紀、本田愛、藤本久貴、中尾武史、橋田徳康、林竜平、相馬剛至、辻川元一、西田幸二
  • Organizer: 第13回再生医療学会総会
  • Place of Presentation: 京都府(口演)
  • Year and Date: 2014-03-05
• [Presentation] タイ王国での角膜上皮再生医療と遠隔診療の実施 (2014)
  • Author(s): 大家義則、小林由紀、本田愛、藤本久貴、中尾武史、橋田徳康、林竜平、相馬剛至、辻川元一、西田幸二
  • Organizer: 角膜カンファランス2014
  • Place of Presentation: 沖縄(ポスター)
  • Year and Date: 2014-01-30
• [Presentation] Ocular surface reconstruction using oral mucosal epithelial cell sheet (2014)
  • Author(s): Yoshinori Oie
  • Organizer: Qatar-Japan Symposium on Tissue Regenerative therapy
  • Place of Presentation: Doha, カタール
  • Year and Date: 2014-01-27
• [Presentation] Development of tissue and cell sheet transportation technique for regenerative medicine. Air transport system of oral mucosal tissue and cell sheets for clinical trial (2013)
  • Author(s): Yoshinori Oie, Satoru Andojo, Natsumi Konno, Hiroshi Takayanagi, Takayuki Nozaki, Shizu Takeda, Takeshi Soma, Motokazu Tsujikawa, Kohji Nishida
  • Organizer: ARVO (the association for research in vision and ophthalmology) annual meeting
  • Place of Presentation: Seattle, アメリカ合衆国(口演)
  • Year and Date: 20130505-09
• [Presentation] Ocular surface reconstruction using human oral mucosal epithelial cell sheets (2013)
  • Author(s): Yoshinori Oie
  • Organizer: The 19^<th> annual meeting of Kyoto Cornea Club 2013
  • Place of Presentation: 京都府(口演)
  • Year and Date: 2013-11-30
• [Presentation] (2013)
  • Author(s): Yoshinori Oie, Takeshi Soma, Ryuhei Hayashi, Motokazu Tsujikawa, Kohji Nishida
  • Organizer: Termis-AP Meeting 2013
  • Place of Presentation: Wuzhen, 中国(口演)
  • Year and Date: 2013-10-25
• [Presentation] 自家培養口腔粘膜上皮細胞シート移植の多施設共同臨床試験 (2013)
  • Author(s): 大家義則
  • Organizer: 第117回日本眼科学会
  • Place of Presentation: 東京都(モーニングセミナー)
  • Year and Date: 2013-04-06
• [Presentation] 臨床試験のための口腔粘膜組織および培養口腔粘膜上皮細胞シートの空輸技術開発 (2013)
  • Author(s): 大家義則、安堵城悟、金野なつみ、高柳泰、野崎貴之、武田志津、林竜平、相馬剛至、辻川元一、西田幸二
  • Organizer: 第117回日本眼科学会
  • Place of Presentation: 東京都(ポスター)
  • Year and Date: 2013-04-05
• [Presentation] 臨床試験を目指した口腔粘膜組織および培養口腔粘膜上皮細胞シートの空輸技術開発 (2013)
  • Author(s): 大家義則、安堵城悟、金野なつみ、高柳泰、野崎貴之、武田志津、林竜平、相馬剛至、辻川元一、西田幸二
  • Organizer: 第12回再生医療学会総会
  • Place of Presentation: 神奈川県(口演)
  • Year and Date: 2013-03-22
• [Presentation] ヒト臨床試験のための培養口腔粘膜上皮細胞シートの輸送技術開発 (2013)
  • Author(s): 大家義則、野崎貴之、金野なつみ、高柳泰、原進、武田志津、林竜平、相馬剛至、辻川元一、西田幸二
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 神奈川県(YIA受賞者講演)
  • Year and Date: 2013-03-21
• [Presentation] Regenerative medicine for cornea (2013)
  • Author(s): Yoshinori Oie
  • Organizer: APAO2013
  • Place of Presentation: Hyderabad, インド(口演)
  • Year and Date: 2013-01-18
• [Presentation] Cell transport system for clinical trial using cultured human oral mucosal epithelial cell sheets (2012)
  • Author(s): Yoshinori Oie、Ryuhei Hayashi、Hiroshi Takayanagi、Susumu Hara、Takayuki Nozaki、Shizu Takeda、Takeshi Soma、Motokazu Tsujikawa、Kohji Nishida
  • Organizer: ISSCR annual meeting
  • Place of Presentation: 神奈川県(ポスター)
  • Year and Date: 2012-06-15
• [Presentation] ヒト臨床試験のための培養口腔粘膜上皮細胞シートの輸送技術開発 (2012)
  • Author(s): 大家義則、林竜平、高柳泰、原進、野崎貴之、武田志津、相馬剛至、辻川元一、西田幸二
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 神奈川県(一般口演)
  • Year and Date: 2012-06-12
• [Presentation] 培養口腔粘膜上皮細胞シートの輸送システム開発 (2012)
  • Author(s): 大家義則、林竜平、高柳泰、原進、野崎貴之、武田志津、相馬剛至、辻川元一、西田幸二
  • Organizer: 角膜カンファランス2012
  • Place of Presentation: 東京都(一般)
  • Year and Date: 2012-02-23
• [Book] "角結膜瘢痕の鑑別", オキュラーサーフェス疾患(眼科臨床エキスパート) (2013)
  • Author(s): 大家義則
  • Total Pages: p. 98-108
  • Publisher: 医学書院
• [Book] 角膜上皮の再生医療 眼科手術 (2013)
  • Author(s): 大家義則
  • Total Pages: 26, p. 553-558
• [Book] "培養上皮細胞シート移植", 角膜・結膜・屈折矯正(眼手術学4) (2013)
  • Author(s): 大家義則
  • Total Pages: p. 97-103
  • Publisher: 文光堂
• [Book] "角膜(総説)", 上皮・感覚器(再生医療叢書4) (2013)
  • Author(s): 大家義則、西田幸二
  • Total Pages: p.26-31
  • Publisher: 朝倉書店
• [Book] "角膜(角膜上皮の再生)", 上皮・感覚器(再生医療叢書4) (2013)
  • Author(s): 大家義則、西田幸二
  • Total Pages: p.39-44
  • Publisher: 朝倉書店