Comprehensive investigation of tension-sensitive factors that related to changes in cytoskeleton and cell adhesion in trabecular meshwork cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 23791994
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Kumamoto University
• Principal Investigator: INOUE Toshihiro 熊本大学, 医学部附属病院, 講師 (00317025)
• Project Period (FY): 2011 – 2013
• Keywords: 緑内障 / 線維柱帯 / 酸化ストレス / 圧感受性

Research Abstract

We made a device to add hydrostatic pressure on cultured cells, resulting in changes in cell morphology even in the control condition, and the data were not analyzable. After treatment of trabecular meshwork cells with Y-27632, 5 genes that were related to antioxidant functions were up-regulated. Of those, the up-regulation of catalase was confirmed by realtime RT-PCR. Pretreatment with Y-27632 increased the signal of WST-8 assay and decreased the signal of DCFDA test reagent under stimulation with hydrogen peroxide. Collectively, it was indicated that ROCK inhibitor induced intracellular expression of antioxidants in the trabecular meshwork cells, and thereby suppressed oxidative stress-induced cell death.

Research Products

• [Journal Article] Epithelial mesenchymal transition-like phenomenon in trabecular meshwork cells (2014)
  • Author(s): Takahashi E, Inoue T, Fujimoto T, Kojima S, Tanihara H
  • Journal Title: Experimental Eye Research Volume: 118 Pages: 72-79
  • DOI: 10.1016/j.exer.2013.11.014
  • Peer Reviewed
• [Journal Article] Oxidative stress response signaling pathways in trabecular meshwork cells and their effects on cell viability (2013)
  • Author(s): Awai-Kasaoka N, Inoue T, Kameda T, Fujimoto T, Inoue-Mochita M, Tanihara H
  • Journal Title: Molecular Vision Volume: 19 Pages: 1332-1340
  • Peer Reviewed
• [Journal Article] Elevated levels of multiple biomarkers of Alzheimer's disease in the aqueous humor of eyes with open-angle glaucoma (2013)
  • Author(s): Inoue T, Kawaji T, Tanihara H
  • Journal Title: Investigative Ophthalmology & Visual Science Volume: 54 Pages: 5353-5358
  • DOI: 10.1167/iovs.13-12245
  • Peer Reviewed
• [Journal Article] The effect of Rho-associated protein kinase inhibitor on monkey Schlemm's canal endothelial cells (2012)
  • Author(s): Kameda T, Inoue T, Inatani M, Fujimoto T, Honjo M, Kasaoka N, Inoue-Mochita M, Yoshimura N, Tanihara H
  • Journal Title: Investigative Ophthalmology & Visual Science Volume: 53 Pages: 3092-3103
  • DOI: 10.1167/iovs.11-8018
  • Peer Reviewed
• [Journal Article] Simultaneous increases in multiple proinflammatory cytokines in the aqueous humor of pseudophakic glaucomatous eyes (2012)
  • Author(s): Inoue T, Kawaji T, Inatani M, Kameda T, Yoshimura N, Tanihara H
  • Journal Title: Journal of Cataract and Refractive Surgery Volume: 38 Pages: 1389-1397
  • DOI: 10.1016/j.jcrs.2012.04.028
  • Peer Reviewed
• [Journal Article] The effect of monocyte chemoattractant protein-1/CC chemokine ligand 2 on aqueous humor outflow facility (2012)
  • Author(s): Tsuboi N, Inoue T, Kawai M, Inoue-Mochita M, Fujimoto T, Awai-Kasaoka N, Yoshida A, Tanihara H
  • Journal Title: Investigative Ophthalmology & Visual Science Volume: 53 Pages: 6702-6707
  • DOI: 10.1167/iovs.12-10376
  • Peer Reviewed
• [Journal Article] Involvement of RhoA/Rho-associated kinase signal transduction pathway in dexamethasone-induced alterations in aqueous outflow (2012)
  • Author(s): Fujimoto T, Inoue T, Kameda T, Awai-Kasaoka N, Inoue-Mochita M, Tsuboi N, Tanihara H
  • Journal Title: Investigative Ophthalmology & Visual Science Volume: 53 Pages: 7097-7108
  • DOI: 10.1167/iovs.12-9989
  • Peer Reviewed
• [Journal Article] Elevated levels of monocyte chemoattractant protein-1 in the aqueous humor after phacoemulsification (2012)
  • Author(s): Kawai M, Inoue T, Inatani M, Tsuboi N, Matsukawa A, Yoshida A, Tanihara H
  • Journal Title: Investigative Ophthalmology & Visual Science Volume: 53 Pages: 7951-7960
  • DOI: 10.1167/iovs.12-10231
  • Peer Reviewed
• [Journal Article] Precise identification of filtration openings on the scleral flap by three-dimensional anterior segment optical coherence tomography (2012)
  • Author(s): Inoue T, Matsumura R, Kuroda U, Nakashima KI, Kawaji T, Tanihara H
  • Journal Title: Investigative Ophthalmology & Visual Science Volume: 53 Pages: 8288-8294
  • DOI: 10.1167/iovs.12-10941
  • Peer Reviewed
• [Journal Article] Monocyte chemotactic protein-1 level in the aqueous humor as a prognostic factor for the outcome of trabeculectomy
  • Author(s): Inoue T, Kawaji T, Tanihara H
  • Journal Title: Clinical and Experimental Ophthalmology Volume: (in press)
  • DOI: 10.1111/ceo.12204
  • Peer Reviewed
• [Presentation] Visualization of actin filament using time-lapse fluorescent microscopy in trabecular meshwork cells (2013)
  • Author(s): Fujimoto T, Inoue T, Inoue-Mochita M, Kasaoka N, Shobayashi K, Tanihara H
  • Organizer: World Glaucoma Congress 2013
  • Place of Presentation: Convention Centre, Vancouver, Canada
  • Year and Date: 20130717-20
• [Presentation] Identification of TGF-β2-induced proinflammatory cytokines secreted from cultured trabecular meshwork cells (2013)
  • Author(s): Inoue-Mochita M, Inoue T, Fujimoto T, Awai-Kasaoka N, Tanihara H
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Washington State Convention Center, Seattle, Washington, USA
  • Year and Date: 20130505-09
• [Presentation] 線維柱帯細胞においてROCK阻害薬が酸化ストレスに与える影響 (2013)
  • Author(s): 笠岡奈々子,井上俊洋,亀田隆範,藤本智和,井上みゆき,谷原秀信
  • Organizer: 第117回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 20130404-07
• [Presentation] Involvement of RhoA/Rho-associated kinase signal transduction pathway in the dexamethasone-induced alterations in aqueous outflow (2012)
  • Author(s): Fujimoto T, Inoue T, Inoue-Mochita M, Kameda T, Kasaoka N, Tanihara H
  • Organizer: European Glaucoma Society 2012
  • Place of Presentation: Tivoli Hotel & Congress Center, Copenhagen, Denmark
  • Year and Date: 20120617-22
• [Presentation] The effect of monocyte chemoattractant protein-1/CC chemokine ligand 2 on the aqueous humor outflow facility (2012)
  • Author(s): Tsuboi N, Inoue T, Fujimoto T, Inoue-Mochita M, Kasaoka N, Kawai M, Shobayashi K, Yoshida A, Tanihara H
  • Organizer: European Glaucoma Society 2012
  • Place of Presentation: Tivoli Hotel & Congress Center, Copenhagen, Denmark
  • Year and Date: 20120617-22
• [Presentation] The effects of Rho/ROCK activation in dexamethasone-induced increase of aqueous-outflow resistance (2012)
  • Author(s): Fujimoto T, Inoue T, Inoue-Mochita M, Kameda T, Kasaoka N, Tanihara H
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Convention Center, Fort Lauderdale, Florida, USA
  • Year and Date: 20120506-10
• [Presentation] The effect of monocyte chemoattractant protein-1/CC chemokine ligand 2 on the aqueous humor outflow facility (2012)
  • Author(s): Tsuboi N, Inoue T, Fujimoto T, Inoue-Mochita M, Kasaoka N, Kawai M, Shobayashi K, Yoshida A, Tanihara H
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Convention Center, Fort Lauderdale, Florida, USA
  • Year and Date: 20120506-10
• [Presentation] The effects of Rho/ROCK activation in dexamethasone induced increase of aqueous outflow resistance (2011)
  • Author(s): Fujimoto T, Inoue T, Inoue-Mochita M, Kameda T, Kasaoka N, Tanihara H
  • Organizer: World Glaucoma Congress 2011
  • Place of Presentation: Palais des Congrès, Paris, France
  • Year and Date: 20110629-0702