2012 Fiscal Year Final Research Report
Eye tissue damage and clinical application of intra vitreous injection
| Project/Area Number |
23791995
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 眼薬理学 / 硝子体注射 / 網膜色素上皮 / TNF-α / 薬物反応性 |
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| Research Abstract |
Retinal pigment epithelial (RPE) cells form a blood-ocular barrier, and their polarized property is crucial for maintaining the barrier functions. Tumor necrosis factor alpha (TNF-α), a major pleotropic inflammatory cytokine that disrupts the barrier function and eventual angiogenesis, is expressed in the choroidal neovascularizations of age-related macular degeneration eyes. Thus, it most likely plays an important role in the progression of the disease. The purpose of this study was to compare the effects of TNF-αon the barrier function of polarized RPE cells. Non-polarized RPE cells were used as negative controls. Isolated porcine RPE cells were seeded on Transwell^ membranes. The polarization of the RPE cells was determined by their high transepithelial electrical resistance and by their differential secretion of vascular endothelial growth factor (lower layer/upper layer >2.5X). Polarized RPE cells were incubated with 10 ng/ml of TNF-αand the TER was measured. TNF-αsignificantly decreased the TER of polarized RPE cells by 17.6 ± 2.7% (P < 0.001) of the control at 24 h and that of non-polarized RPE cells by 5.4 ± 6.5% (P = 0.401). The p38 mitogen-activated protein kinase (MAPK) inhibitor, SB203580, blocked the effects of TNF-αof decreasing the TER. Cell junction-related molecules, e.g., ZO-1, located between cells in control RPE cells, were disassembled by TNF-α, and this breakdown was suppressed by SB203580 in polarized RPEs. These results indicate that the breakdown of the RPE barrier function was caused exclusively by TNF-αin polarized RPEs, and TNF-αwas acting through the p38 MAPK pathways. Investigations of polarized RPE cells should be more suitable for in vitro studies of the pathophysiology of retinochoroidal diseases.
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[Journal Article]
Author(s)
Shirasawa M, Sonoda S, Terasaki H, Arimura N, Otsuka H, Yamashita T, Uchino E, Hisatomi T, Ishibashi T, Sakamoto T
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Journal Title
DOI
Peer Reviewed
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