2013 Fiscal Year Final Research Report
Roles of soluble VEGF receptor on angiogenesis of eye in vivo
| Project/Area Number |
23792017
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
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| Research Collaborator |
HATTA Toshihisa 金沢医科大学, 医学部, 教授 (20238025)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 可溶型Flt-1 / HMVEC / RNA結合タンパク質 |
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| Research Abstract |
Here, we showed the mechanisms for regulating alternative 3'-end processing of soluble Flt-1, a potential antiangiogenic factor. We found that hnRNP D down-regulated soluble Flt-1 expression by binding to the regulatory sequence on intron 13 of Flt-1 pre-mRNA. Furthermore, we carried out the lentiviral mediated transgenesis by in vivo manipulation of spermatogonical stem cell. It was suggested that the method was not effective because lentiviral particle couldn't infect to spermatogonical stem cell. Therefore, we are going to create a transgenic mouse conventionally and show the roles of soluble Flt-1 on angiogenesis of eye in vivo.
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Research Products
(19 results)
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[Journal Article] Identification of a major enzyme for the synthesis and hydrolysis of cyclic ADP-ribose in amphibian cells and evolutional conservation of the enzyme from human to invertebrate2012
Author(s)
Ikeda, T., Takasawa, S., Noguchi, N., Nata, K., Yamauchi, A., Takahashi, I., Yoshikawa, T., Sugawara, A., Yonekura, H. and Okamoto, H.
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Journal Title
Molecular and Cellular Biochemistry
Volume: 366(1-2)
Pages: 69-80
DOI
Peer Reviewed
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[Journal Article] Hypoxia down-regulates sFlt-1 (sVEGFR-1) expression in human microvascular endothelial cells by a mechanism involving mRNA alternative processing2011
Author(s)
Ikeda, T., Sun, L., Tsuruoka, N., Ishigaki, Y., Yoshitomi, Y., Yoshitake, Y. and Yonekura, H.
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Journal Title
Biochemical Journal
Volume: 366(1-2)
Pages: 69-80
DOI
Peer Reviewed
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[Journal Article] Identification of the COL2A1 mutation in type I Stickler syndrome patients using RNA from freshly isolated peripheral white blood cells2011
Author(s)
Yaguchi, H., Ikeda, T., Osada, H., Yoshitake, Y., Sasaki, H. and Yonekura, H.
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Journal Title
Genetic Testing and Molecular Biomarkers
Volume: 15(4)
Pages: 231-237
DOI
Peer Reviewed
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[Journal Article] Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells2011
Author(s)
Osada, H., Yoshitake, Y., Ikeda, T., Ishigaki, Y., Takata, T., Tomosugi, N., Sasaki, H. and Yonekura, H.
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Journal Title
Molecular Vision
Volume: 17(17-20)
Pages: 159-169
DOI
Peer Reviewed
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[Presentation] Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells2012
Author(s)
Osada, H., Yoshitake, Y., Ikeda, T., Ishigaki, Y., Takata, T., Tomosugi, N., Kubo, E., Sasaki, H. and Yonekura, H.
Organizer
International Conference on The Lens
Place of Presentation
Hawaii
Year and Date
20120115-20
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[Presentation] Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells2011
Author(s)
Yoshitake, Y., Osada, H., Ikeda, T., Yoshitomi, Y., Ishigaki, Y., Sasaki, H. and Yonekura, H.
Organizer
36th FEBS Congress
Place of Presentation
Torino, Italy
Year and Date
20110625-30
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