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2013 Fiscal Year Final Research Report

Roles of soluble VEGF receptor on angiogenesis of eye in vivo

Research Project

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Project/Area Number 23792017
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ophthalmology
Research InstitutionKanazawa Medical University

Principal Investigator

IKEDA Takayuki  金沢医科大学, 医学部, 講師 (00374942)

Research Collaborator HATTA Toshihisa  金沢医科大学, 医学部, 教授 (20238025)
Project Period (FY) 2011 – 2013
Keywords可溶型Flt-1 / HMVEC / RNA結合タンパク質
Research Abstract

Here, we showed the mechanisms for regulating alternative 3'-end processing of soluble Flt-1, a potential antiangiogenic factor. We found that hnRNP D down-regulated soluble Flt-1 expression by binding to the regulatory sequence on intron 13 of Flt-1 pre-mRNA. Furthermore, we carried out the lentiviral mediated transgenesis by in vivo manipulation of spermatogonical stem cell. It was suggested that the method was not effective because lentiviral particle couldn't infect to spermatogonical stem cell. Therefore, we are going to create a transgenic mouse conventionally and show the roles of soluble Flt-1 on angiogenesis of eye in vivo.

  • Research Products

    (19 results)

All 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (14 results) Remarks (1 results)

  • [Journal Article] Identification of a major enzyme for the synthesis and hydrolysis of cyclic ADP-ribose in amphibian cells and evolutional conservation of the enzyme from human to invertebrate2012

    • Author(s)
      Ikeda, T., Takasawa, S., Noguchi, N., Nata, K., Yamauchi, A., Takahashi, I., Yoshikawa, T., Sugawara, A., Yonekura, H. and Okamoto, H.
    • Journal Title

      Molecular and Cellular Biochemistry

      Volume: 366(1-2) Pages: 69-80

    • DOI

      10.1007/s11010-012-1284-0

    • Peer Reviewed
  • [Journal Article] Hypoxia down-regulates sFlt-1 (sVEGFR-1) expression in human microvascular endothelial cells by a mechanism involving mRNA alternative processing2011

    • Author(s)
      Ikeda, T., Sun, L., Tsuruoka, N., Ishigaki, Y., Yoshitomi, Y., Yoshitake, Y. and Yonekura, H.
    • Journal Title

      Biochemical Journal

      Volume: 366(1-2) Pages: 69-80

    • DOI

      10.1042/BJ20101490

    • Peer Reviewed
  • [Journal Article] Identification of the COL2A1 mutation in type I Stickler syndrome patients using RNA from freshly isolated peripheral white blood cells2011

    • Author(s)
      Yaguchi, H., Ikeda, T., Osada, H., Yoshitake, Y., Sasaki, H. and Yonekura, H.
    • Journal Title

      Genetic Testing and Molecular Biomarkers

      Volume: 15(4) Pages: 231-237

    • DOI

      10.1089/gtmb.2010.0138

    • Peer Reviewed
  • [Journal Article] Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells2011

    • Author(s)
      Osada, H., Yoshitake, Y., Ikeda, T., Ishigaki, Y., Takata, T., Tomosugi, N., Sasaki, H. and Yonekura, H.
    • Journal Title

      Molecular Vision

      Volume: 17(17-20) Pages: 159-169

    • DOI

      10.3892/ijo_00000707

    • Peer Reviewed
  • [Presentation] 神経—血管相互作用を介した血管ネットワーク形成におけるJunBの機能2013

    • Author(s)
      吉冨泰央、池田崇之、吉竹佳の、八田稔久、加藤伸郎、米倉秀人
    • Organizer
      金沢医科大学医学会第47回学術集会
    • Place of Presentation
      石川県内灘
    • Year and Date
      2013-07-06
  • [Presentation] Increased expression of Epac2 during in vitro tube formation in human microvascular endothelial cells2012

    • Author(s)
      Yoshitake, Y., Ikeda, T., Yoshitomi, Y. and Yonekura, H.
    • Organizer
      37th FEBS Congress
    • Place of Presentation
      Seville, Spain
    • Year and Date
      20120904-09
  • [Presentation] 血管内皮細胞における可溶型Flt-1(可溶型VEGF受容体-1)mRNA選択的3'端プロセシング機構の解析2012

    • Author(s)
      池田崇之、吉冨泰央、石垣靖人、吉竹佳の、米倉秀人
    • Organizer
      第14回日本RNA学会
    • Place of Presentation
      仙台
    • Year and Date
      20120718-20
  • [Presentation] Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells2012

    • Author(s)
      Osada, H., Yoshitake, Y., Ikeda, T., Ishigaki, Y., Takata, T., Tomosugi, N., Kubo, E., Sasaki, H. and Yonekura, H.
    • Organizer
      International Conference on The Lens
    • Place of Presentation
      Hawaii
    • Year and Date
      20120115-20
  • [Presentation] 毛細血管内皮細胞のマトリゲル上での管腔形成過程ではEpac2の発現が上昇する2012

    • Author(s)
      吉竹佳の、池田崇之、吉冨泰央、田中一美、米倉秀人
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-16
  • [Presentation] Flt-1(VEGF受容体-1)mRNA選択的3'端プロセシング機構の解析2012

    • Author(s)
      池田崇之、吉冨泰央、吉竹佳の、米倉秀人
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-15
  • [Presentation] 低酸素状態はmRNA選択的3'端プロセシングを介して微小血管内皮細胞の可溶型VEGF受容体(可溶型Flt-1)産生を抑制する2012

    • Author(s)
      池田崇之、吉冨泰央、石垣靖人、吉竹佳の、米倉秀人
    • Organizer
      日本生化学会北陸支部第30回記念大会
    • Place of Presentation
      金沢
    • Year and Date
      2012-05-26
  • [Presentation] 神経—血管相互作用により血管で活性化される遺伝子群の同定とその機能解析2011

    • Author(s)
      吉冨泰央、池田崇之、吉竹佳の、加藤伸郎、米倉秀人
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      20110921-24
  • [Presentation] Hypoxia doun-regulates sFlt-1 (sVEGFR-1) expression in human microvascular endothelial cells by a mechanism involving mRNA alternative processing2011

    • Author(s)
      Ikeda, T., Yoshitomi, Y., Ishigaki, Y., Yoshitake, Y. and Yonekura, H.
    • Organizer
      36th FEBS Congress
    • Place of Presentation
      Torino, Italy
    • Year and Date
      20110625-30
  • [Presentation] Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells2011

    • Author(s)
      Yoshitake, Y., Osada, H., Ikeda, T., Yoshitomi, Y., Ishigaki, Y., Sasaki, H. and Yonekura, H.
    • Organizer
      36th FEBS Congress
    • Place of Presentation
      Torino, Italy
    • Year and Date
      20110625-30
  • [Presentation] 低酸素刺激による微小血管内皮細胞の可溶型VEGF受容体(可溶型Flt-1)産生の抑制2011

    • Author(s)
      池田崇之、吉冨泰央、石垣靖人、吉竹佳の、米倉秀人
    • Organizer
      RNAフロンティアミーティング
    • Place of Presentation
      愛知県大府
    • Year and Date
      2011-08-31
  • [Presentation] 低酸素刺激による微小血管内皮細胞の可溶型VEGF受容体(可溶型Flt-1)産生の抑制2011

    • Author(s)
      池田崇之、吉冨泰央、石垣靖人、吉竹佳の、米倉秀人
    • Organizer
      金沢医科大学医学会第47回学術集会
    • Place of Presentation
      石川県内灘
    • Year and Date
      2011-07-09
  • [Presentation] 低酸素刺激による微小血管内皮細胞の可溶型VEGF受容体(可溶型Flt-1)産生の抑制2011

    • Author(s)
      池田崇之
    • Organizer
      日本生化学会北陸支部第29回大会
    • Place of Presentation
      金沢
    • Year and Date
      2011-05-28
  • [Presentation] Soluble VEGFR-1 production is down-regulated under hypoxia in human microvascular endothelial cells by a mechanism involving alternative mRNA 3′-end processing2011

    • Author(s)
      Ikeda, T., Yoshitomi, Y., Ishigaki, Y., Yoshitake, Y. and Yonekura, H.
    • Organizer
      The Molecular Biology Society of Japan The 11th Spring Symposium International Symposium on Tumor Biology in Kanazawa
    • Place of Presentation
      金沢
    • Year and Date
      2011-05-25
  • [Remarks]

    • URL

      http://www.kanazawa-med.ac.jp/~biochem/

URL: 

Published: 2015-06-25  

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