2013 Fiscal Year Final Research Report
Mesenchymal stem cell transplantation in a nitrofen-induced congenital diaphragmatic hernia rat model
| Project/Area Number |
23792031
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatric surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
NAGATA Kouji 九州大学, 大学病院, 助教 (20419568)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 再生医療 / 細胞治療 / 間葉系幹細胞 / 肺低形成 |
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| Research Abstract |
The aim of this study was to evaluate the efficacy of MSCs in a nitrofen-induced CDH rat model.Pregnant rats were exposed to nitrofen on E9.5. MSCs were isolated from the eGFP transgenic rat lungs. The MSCs were transplanted into the nitrofen-induced E12.5 rats via the uterine vein, and the E21 lung explants were harvested. The study animals were divided into three: the control group, the CDH group, and the MSC-treated CDH group. The specimens were morphologically analyzed using HE and immunohistochemical staining. The alveolar and medial walls of the pulmonary arteries were significantly thinner in the MSC-treated CDH group than in the CDH group. The alveolar air space areas were larger than in the CDH group. MSC transplantation potentially promotes alveolar and pulmonary artery development, thereby reducing the severity of pulmonary hypoplasia.
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[Journal Article] The outcome of patients with congenital diaphragmatic hernia and having indications for a Fontan operation : Results of a national survey in Japan2014
Author(s)
Shiono N, Inamura N, Takahashi S, Nagata K, Fujino F, Hayakawa M, Usui N, Okuyama H, Kanamori Y, Taguchi T, Minakami H
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Journal Title
Pediatr Surg Int
Volume: (Accept In press)
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