Searching for new treatment of Hirschspurung disease by using newly developed mice model

2013 Fiscal Year Final Research Report

• Project/Area Number: 23792033
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: Juntendo University
• Principal Investigator: TANAKA Nana 順天堂大学, 医学部, 助教 (50530656)
• Project Period (FY): 2011 – 2013
• Keywords: ヒルシュスプルング病 / 神経堤細胞 / マウスモデル / 蛍光イメージング

Research Abstract

Disruption of vagal enteric neural crest cells(NCC) migration appears to induce sacral NCC activation in the anorectum, which could have implications for the etiology of the thick nerve fibers seen in Hirschsprung disease(HD) anorectum, which could in fact be a secondary phenomenon.
To establish new treatment for HD, we examined the effect of laminin-1 in NCC migration using SOX10-Venus mice gut. Our results suggest that laminin promotes NCC migration in mice.

Research Products

• [Journal Article] Laminin-1 promotes enteric nervous system development in mouse embryo (2013)
  • Author(s): Nakazawa N, Miyahara K, Okawada M, Yamataka A, Suzuki R, Akazawa C, Tomikawa-Ichikawa N, Arikawa-Hirasawa E
  • Journal Title: Pediatr Surg Int Volume: 29(11) Pages: 1205-1208
• [Journal Article] Anorectal neural crest derived cell behavior after the migration of vagal neural crest derived cells is surgically disrupted : implications for the etiology of Hirschsprung's disease (2013)
  • Author(s): Miyahara K, Kato Y, Suzuki R, Akazawa C, Tanaka N, Koga H, Doi T, Lane GJ, Yamataka A
  • Journal Title: Pediatr Surg Int Volume: 29(1) Pages: 9-12
• [Presentation] Laminin-1 promotes neuronal development in mouse embryonic gut (2013)
  • Author(s): N Nakazawa, K Miyahara, M Okawada, R Suzuki, M Urao, C Akazawa, A Yamataka, E Arikawa-Hirasawa
  • Organizer: 第50回日本小児外科学会
  • Place of Presentation: 東京
  • Year and Date: 20130000
• [Presentation] Anorectal neural crest derived cell behavior after the migration of vagal neural crest derived cells is surgically disrupted : Implications for the etiology of Hirschsprung's disease (2012)
  • Author(s): N Nakazawa, K Miyahara, R Suzuki, Y Kato, GJ Lane, C Akazawa, H Koga, T Doi, A Yamataka
  • Organizer: 25th International symposium on paediaric surgical research
  • Place of Presentation: London, UK
  • Year and Date: 20120921-22
• [Presentation] Laminin-1 promotes neuronal development in mouse embryonic gut (2012)
  • Author(s): N Nakazawa, K Miyahara, M Okawada, Y Liu, C Akazawa, A Yamataka, E Arikawa-Hirasawa
  • Organizer: 25th International symposium on paediaric surgical research
  • Place of Presentation: London, UK
  • Year and Date: 20120921-22
• [Remarks] Aesculap Prize (Second Prize) at the 25^<th> International Symposium of Paediatric Surgical Research, London, UK, 2012