Effect of the treatment for the anal canal dysfunction using dedifferentiated fat cell(DFAT)

2012 Fiscal Year Final Research Report

• Project/Area Number: 23792034
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: Nihon University
• Principal Investigator: KANEDA Hide 日本大学, 医学部, 専修指導医 (30598967)
• Project Period (FY): 2011 – 2012
• Keywords: 先天性消化器疾患学

Research Abstract

I found significantlyα-Smooth muscle actin (αSMA) positive cell at the smooth muscle differentiation medium compared with the control (5%FCS), cultured rat DFAT with the smooth muscle differentiation medium (5%FCS, TGFβ5ng/ml). To confirm whether rat DFAT have possibility of smooth muscle and skeletal muscle differentiation in vivo, I producted anal canal (it consists of smooth muscle and skeletal muscle) dysfunction rat model using cardiotoxin (CTX). Green fluorescence protein (GFP)-labeled DFAT or PBS were injected into para-anal canal tissue. Immunohistochemistry revealed that inner smooth muscle and external striated muscle layers in anal canal showed atrophy or necrosis in the control group, whereas DFAT transplantation led an increase of smoothe muscle and striated muscle mass with variable fiber orientation. GFP-labeled DFAT model showed a decrease of inflammatory cells in HE stain. DFAT cell transplantation resulted in a significant improvement of anal canal pressure, compared with control. DFAT cell transplantation promotes anal canal muscle regeneration and improves anal canal pressure.

Research Products

• [Presentation] 脱分化成熟脂肪細胞(DFAT)のT細胞増殖抑制効果とマウス皮膚移植の急性拒絶期における免疫抑制効果 (2012)
  • Author(s): 細川崇,杉藤公信,小沼憲祥,石岡茂樹,金田英秀,細田利史,大橋研介,池田太郎,越永従道,松本太郎,加野浩一郎
  • Organizer: 第49回日本小児外科学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20120514-0516
• [Presentation] 脱分化脂肪細胞(dedifferentiated fat cells:DFAT)を用いた炎症性腸疾患への治療検討 (2012)
  • Author(s): 石岡茂樹,細川崇,小沼憲祥,杉藤公信,池田太郎,金田英秀,細田利史,古屋武史,小宮山翔吾,入部雄司,越永従道,加野浩一郎,松本太郎
  • Organizer: 第49回日本小児外科学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20120514-0516
• [Presentation] マウス皮膚移植における脱分化成熟脂肪細胞(DFAT)の免疫抑制効果 (2012)
  • Author(s): 小沼憲祥,石岡茂樹,細川たかし,池田太郎,杉藤公信,古屋武史,金田英秀,南郷容子,小宮山翔吾,越永従道,加納浩一郎,松本太郎
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 千葉
  • Year and Date: 20120412-0414
• [Presentation] マウス炎症性腸疾患モデルに対する脱分化脂肪細胞(DFAT)の効果 (2012)
  • Author(s): 石岡茂樹,細川崇,小沼憲祥,杉藤公信,池田太郎,金田英秀,細田利史,古屋武史,小宮山翔吾,入部雄司,越永従道,加野浩一郎,松本太郎
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 千葉
  • Year and Date: 20120412-0414