Analysis of the premature birth-induced mechanism by the periodontal disease bacteria - cytomegalovirus superinfection model

2013 Fiscal Year Final Research Report

• Project/Area Number: 23792102
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Osaka University
• Principal Investigator: INABA Hiroaki 大阪大学, 歯学研究科(研究院), 助教 (70359850)
• Project Period (FY): 2011 – 2012
• Keywords: P. gingivalis / 早産 / シグナル伝達 / アポトーシス / 細胞周期

Research Abstract

P. gingivalis activates ERK1/2-Ets1 and the cellular DNA damage signaling pathways that act through an ATR/Chk2/p53-dependent pathway to cause G1 arrest and apoptosis in human trophoblast cells. Furthermore, P. gingivalis gingipain proteases degrade MDM2, which is the p53 degradation-related protein, thus contributing to p53 accumulation, G1 phase cell cycle arrest and apoptosis.

Research Products

• [Journal Article] Porphyromonas gingivalis promotes invasion of oral squamous cell carcinoma through induction of proMMP9 and its activation (2014)
  • Author(s): Inaba H、Sugita H、Kuboniwa M、Iwai S、Hamada M、Noda T、Morisaki I、Lamont RJ、Amano A
  • Journal Title: Cellular Microbiology Volume: 16巻1号 Pages: 131-145
  • DOI: 10.1111/cmi.12211
  • Peer Reviewed
• [Journal Article] Distribution and molecular characterization of Porphyromonas gulae carrying a new fimA genotype (2012)
  • Author(s): Yamasaki Y、Nomura R、Nakano K、Inaba H、Kuboniwa M、Shirai M、Kato Y、Murakami M、Naka S、Matsumoto-Nakano M、Ooshima T、Amano A、Asai F
  • Journal Title: Veterinary Microbiology Volume: 161巻1-2号 Pages: 196-205
  • DOI: 10.1016/j.vetmic.2012.07.026
  • Peer Reviewed
• [Journal Article] Identification of signaling pathways mediating cell cycle arrest and apoptosis induced by Porphyromonas gingivalis in human trophoblasts (2012)
  • Author(s): Inaba H、Kuboniwa M、Sugita H、Lamont RJ、Amano A
  • Journal Title: Infection and Immunity Volume: 80巻8号 Pages: 2847-2857
  • DOI: 10.1128/IAI.00258-12
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalis SerB mediated dephosphorylation of host cell cofilin modulates invasion efficiency (2012)
  • Author(s): Moffatt C、Inaba H、Hirano T、Lamont RJ
  • Journal Title: Cellular Microbiology Volume: 14巻1号 Pages: 577-588
  • DOI: 10.1111/j.1462-5822.2011.01743.x
  • Peer Reviewed
• [Journal Article] Proliferation of smooth muscle cells stimulated by Porphyromonas gingivalis is inhibited by apple polyphenol (2011)
  • Author(s): Inaba H、Tagashira M、Kanda T、Amano A
  • Journal Title: Journal of Periodontology Volume: 82巻11号 Pages: 1616-1622
  • DOI: 10.1902/jop.2011.100785
  • Peer Reviewed
• [Journal Article] Characterization of aortic aneurysms in cardio- vascular disease patients harboring Porphyromonas gingivalis (2011)
  • Author(s): Nakano K、Wada K、Nomura R、Nemoto H、Inaba H, Kojima A、Naka S、Hokamura K、Mukai T、Nakajima A、Umemura K、Kamisaki Y、Yoshioka H、Taniguchi K、Amano A、Ooshima T
  • Journal Title: Oral disease Volume: 17巻4号 Pages: 370-378
  • DOI: 10.1111/j.1601-0825.2010.01759.x
  • Peer Reviewed
• [Presentation] 感染と全身疾患を結ぶ分子基盤の探索 (2014)
  • Author(s): 稲葉裕明、P. gingivalis
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
  • Year and Date: 20140300
• [Presentation] Porphyromonas gingivalis promotes invasion of oral cancer through MMP9 activation (2013)
  • Author(s): Inaba H、Amano A
  • Organizer: 10th World Congress on Preventive Dentistry
  • Place of Presentation: ハンガリー・ブダペスト
  • Year and Date: 20131000
• [Presentation] 歯肉上皮細胞由来ポリアミンがPorphyromonas gingivalisの病原性に及ぼす影響 (2013)
  • Author(s): 久保庭雅恵、Alghamdi Samar、稲葉裕明、橋野恵衣、天野敦雄
  • Organizer: 第62回口腔衛生学会・総会
  • Place of Presentation: 長野・松本
  • Year and Date: 20130500
• [Presentation] Porphyromonas gingivalis感染がヒト口腔癌細胞の浸潤能に及ぼす影響 (2013)
  • Author(s): 稲葉裕明、久保庭雅恵、天野敦雄
  • Organizer: 第62回口腔衛生学会・総会
  • Place of Presentation: 長野・松本
  • Year and Date: 20130500
• [Presentation] Multiple pathways regulate apoptosis induced by periodontal pathogen in tropho- blast (2012)
  • Author(s): Inaba H、Kuboniwa M、Amano A
  • Organizer: 第60回JADR
  • Place of Presentation: 新潟
  • Year and Date: 20121200
• [Presentation] ヒト胎盤栄養膜細胞におけるP. gingivalis感染がDNA損傷シグナルに及ぼす影響 (2012)
  • Author(s): 稲葉裕明、久保庭雅恵、天野敦雄
  • Organizer: 第54回歯科基礎医学会総会
  • Place of Presentation: 福島・郡山
  • Year and Date: 20120900
• [Presentation] 歯肉上皮細胞p53タンパクへのPorphyromonas gingivalis感染の影響 (2011)
  • Author(s): 杉田英之、稲葉裕明、天野敦雄、森崎市治郎
  • Organizer: 第28回日本障害者歯科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 20111100
• [Presentation] Multiple signalling pathways are involved in apoptosis and cell cycle arrest induced by periodontal pathogen in human trophoblast (2011)
  • Author(s): Inaba H、Kuboniwa M、Sugita H、Amano A
  • Organizer: 第3回EMBO meeting
  • Place of Presentation: オーストリア・ウィーン
  • Year and Date: 20110900