A study to develop a novel therapeutic/ preventive strategy for oral bacterial infectious diseases based on the obstruction of lipoprotein modification enzymes

2012 Fiscal Year Final Research Report

• Project/Area Number: 23792113
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Showa University
• Principal Investigator: TAKAFUMI Arimoto 昭和大学, 歯学部, 講師 (60407393)
• Project Period (FY): 2011 – 2012
• Keywords: lipoprotein / prolipoprotein diacylglyceryl transferase / Streptococcus mutans / signal peptidase

Research Abstract

We have been engaged in the work regarding surface lipoprotein on cariogenic bacterium, Streptococcus mutans. Until now, we have identified and functional analyzed lipoproteins related to sugar and amino acid uptake. Moreover, these lipoproteins have been clarified to be modified by lipoprotein modification enzymes, prolipoprotein diacylglyceryl transferase (Lgt) and lipoprotein-specific signal peptidase II (LspA), which is very important for the pysiological function of the lipoproteins. In this study, we identified that lipoprotein OpcC was involved in the acid tolerance which is one of cariogenic properties of S. mutans and demonstrated that the lipoprotein OpcC was under the control of Lgt and LspA in the localization and physiological function. Accumulated evidence and this finding strongly suggest that lipoproteins are definitely related to the pathogenic properties of S. mutans. Furthermore, lipoprotein modification enzymes should be attractive targets to develop a new strategy for prevention and therapeutic against dental caries.

Research Products

• [Journal Article] Involvement of lipoprotein PpiA of Streptococcus gordonii in evasion of phagocytosis by macrophages (2013)
  • Author(s): Kasei Cho, Takafumi Arimoto, Takeshi Igarashi, Matsuo Yamamoto
  • Journal Title: Molecular Oral Microbiology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Relationship between CiaRH and Lipoprotein OpcC in the Acid Tolerance of Streptococcus mutans (2013)
  • Author(s): Taniguchi, Makoto; Arimoto, Takafumi; Igarashi, Takeshi
  • Journal Title: Dental Medicine Research Volume: 33 (1) Pages: 2-11
  • Peer Reviewed
• [Journal Article] Toll-like receptor 2-mediated modulation of growth and functions of regulatory T cells by oralstreptococci (2013)
  • Author(s): Saeki, Ayumi; Segawa, Taku; Abe, Tsugumi; Sugiyama, Masahiro; Arimoto, Takafumi; Hara, Hiroshi; Hasebe, Akira; Ohtani, Makoto; Tanizume, Naoho; Ohuchi, Manabu; Kataoka, Hideo; Kawanami, Masamitsu; Yokoyama, Atsuro; Ken-ichiro
  • Journal Title: Molecular Oral Microbiology Volume: (In press)
  • Peer Reviewed
• [Presentation] Relationship between lipoprotein PpiA and scavenger receptors in Streptococcus gordonii evasion of phagocytosis (2012)
  • Author(s): K. Chou, T. Arimoto, M. Taniguchi, T. Ando, M. Yamamoto, T. Igarashi
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 20120327-29
• [Presentation] Modulation of growth and functions of CD4+CD25+Foxp3+ regulatory T cells by oral streptococci (2012)
  • Author(s): A. Saeki, T. Segawa, H. Kataoka, A. Hasebe, T. Arimoto, ・・・K. Shibata
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 20120327-29
• [Presentation] Roles of lipoproteins in clearance of oral streptococci (2012)
  • Author(s): T. Segawa, A. Hasebe, A. Saeki, H. Kataoka, T. Arimoto, ・・・K. Shibata
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 20120327-29
• [Remarks]
  • URL: http://www.showa-u.ac.jp/sch/dent/major/dbisei/index.html