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2012 Fiscal Year Final Research Report

Role of Sox9 transcriptional network during chondrocyte differentiation

Research Project

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Project/Area Number 23792121
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional basic dentistry
Research InstitutionOsaka University

Principal Investigator

HATA Kenji  大阪大学, 歯学研究科, 准教授 (80444496)

Co-Investigator(Renkei-kenkyūsha) NISHIMURA Riko  大阪大学, 歯学研究科, 教授 (60294112)
Project Period (FY) 2011 – 2012
Keywords軟骨細胞 / Sox9 / 転写因子
Research Abstract

Sox9 is a transcription factor critical for endochondral ossification; however, proof of its epigenetic regulation remains elusive. Here, we identified Arid5b (AT-rich interactive domain 5b) as a novel transcriptional co-regulator for Sox9. Arid5b physically interacted with Sox9 and cooperatively promoted chondrogenesis through direct binding to the Col2a1gene promoter. Arid5b^-/-mice showed growth retardation with delayed chondrogenesis. The methylation level of H3K9 in chondrogenic marker gene promoters was markedly increased in Arid5b^-/- chondrocytes. In conclusion, our findings establish a novel epigenomic mechanism of skeletal development, where Arid5b promotes chondrogenesis by facilitating a relationship between Sox9 and Phf2-mediated histone demethylation of chondrogenic gene promoters.

  • Research Products

    (14 results)

All 2013 2012 2011

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (8 results)

  • [Journal Article] Spatiotemporal disorder in the axial skeleton development of theMesp2-null mouse: a model of spondylocostal dysostosis and spondylothoracicdysostosis2013

    • Author(s)
      Makino Y, Takahashi Y, Tanabe R, Tamamura Y, Watanabe T, Haraikawa M, Hamagaki M, Hata K,Kanno J, Yoneda T.Saga Y, Goseki-Sone M, Kaneko K, Yamaguchi A,Iimura T
    • Journal Title

      Bone

      Volume: 53(1) Pages: 248-58

    • Peer Reviewed
  • [Journal Article] Retinoic acid regulates commitment of undifferentiated mesenchymal stem cells into osteoblasts and adipocytes2013

    • Author(s)
      Hisada K, Hata K,Ichida F, Matsubara T, Orimo H, Nakano T, Yatani H, Nishimura R, Yoneda T.
    • Journal Title

      J Bone Miner Metab

      Volume: 31(1) Pages: 53-63

    • Peer Reviewed
  • [Journal Article] Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification2012

    • Author(s)
      Nishimura R, Wakabayashi M, Hata K, Matsubara T, Honma S, Wakisaka S, Kiyonari H, Shioi G, Yamaguchi A, Tsumaki N, Akiyama H, Yoneda T
    • Journal Title

      J Biol Chem

      Volume: 287(40) Pages: 33179-90

    • Peer Reviewed
  • [Journal Article] Regulation of endochondral ossification by transcription factors2012

    • Author(s)
      Nishimura R, Hata K, Ono K, Amano K, Takigawa Y, Wakabayashi M, Takashima R, Yoneda T.
    • Journal Title

      Front Biosci

      Volume: 17 Pages: 2657-66

    • Peer Reviewed
  • [Journal Article] Regulation of bone and cartilage development by network between BMP signalling and transcription factors2012

    • Author(s)
      Nishimura R, Hata K, Matsubara T, Wakabayashi M, Yoneda T
    • Journal Title

      J Biochem

      Volume: 151(3) Pages: 247-54

    • Peer Reviewed
  • [Journal Article] Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription2011

    • Author(s)
      Amano K, Hata K, Muramatsu S, Wakabayashi M, Takigawa Y, Ono K, Nakanishi M,Takashima R, Kogo M, Matsuda A, Nishimura R, Yoneda T
    • Journal Title

      Mol Biol Cell

      Volume: 22(8) Pages: 1300-11

    • Peer Reviewed
  • [Presentation] The transcription factor FoxC1 regulates chondrogenesis together with Gli2 through induction of PTHrP2012

    • Author(s)
      Yoshida M,Hata K, Takashima R, Iseki S, Takano-Yamamoto T, Nishimura R and Yoneda T
    • Organizer
      第35回米国骨代謝学会
    • Place of Presentation
      ミネアポリス
    • Year and Date
      20121012-15
  • [Presentation] 転写因子 Arid5b は Sox9 標的遺伝子プロモーター領域のヒストン脱メチル化を促進し軟骨細胞分化を制御する2012

    • Author(s)
      波多賢二、高島利加子、前田芳信、西村理行、米田俊之
    • Organizer
      第54回歯科基礎医学会
    • Place of Presentation
      福島
    • Year and Date
      20120925-27
  • [Presentation] Forkhead box transcription factor FoxC1 controls endochondral bone formation through directly regulating PTHrP expression2012

    • Author(s)
      Yoshida M,Hata K, Takashima R, Iseki S, Takano-Yamamoto T, Nishimura R and Yoneda T
    • Organizer
      オーストラリア-ニュージーランド骨代謝学会
    • Year and Date
      20120902-05
  • [Presentation] 転写因子 FoxC1 は PTHrP の発現誘導を介して内軟骨性骨形成を制御する2012

    • Author(s)
      吉田倫子,波多賢二,高島利加子,井関祥子,山本照子,西村理行,米田俊之
    • Organizer
      第30回日本骨代謝学会
    • Place of Presentation
      東京
    • Year and Date
      20120719-21
  • [Presentation] The transcription factor Arid5b is a novel partner of Sox9 in endochondral bone formationR Takashima2011

    • Author(s)
      K Hata, K Ono, M Wakabayashi, K Amano, M Nakanishi, Y Maeda, R Whitson,R Nishimura, T Yoneda
    • Organizer
      第34回米国骨代謝学会
    • Place of Presentation
      サンディエゴ
    • Year and Date
      20110916-20
  • [Presentation] The transcription factor Arid5b is a novel partner of Sox9 in endochondral bone formation2011

    • Author(s)
      R Takashima, K Hata, K Ono, M Wakabayashi, K Amano, M Nakanishi, Y Maeda, R Whitson,R Nishimura, T Yoneda
    • Organizer
      the ANZBMS 21st Annual Scientific Meeting
    • Place of Presentation
      ゴールドコースト
    • Year and Date
      20110904-06
  • [Presentation] Transcriptional regulation of endochondral ossification2011

    • Author(s)
      Kenji Hata
    • Organizer
      第29回日本骨代謝学会
    • Place of Presentation
      国際シンポジウム(招待講演),大阪
    • Year and Date
      20110719-21
  • [Presentation] 国際シンポジウム(招待講演)2011

    • Author(s)
      波多賢二,高島利加子,西村理行,米田俊之
    • Organizer
      第29回日本骨代謝学会
    • Place of Presentation
      大阪 招待講演
    • Year and Date
      20110719-21

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Published: 2014-08-29   Modified: 2014-10-21  

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