2012 Fiscal Year Final Research Report
Involvement of a novel molecule in the phospho-regulation of exocytosis using a cell-based model
Project/Area Number |
23792127
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Functional basic dentistry
|
Research Institution | Kyushu University |
Principal Investigator |
GAO Jing 九州大学, 歯学研究院, 助教 (40585882)
|
Project Period (FY) |
2011 – 2012
|
Keywords | 開口分泌 / リン酸化 / SNARE / ホスファターゼ |
Research Abstract |
In the current study, we investigated the involvement of phospholipase C-related but catalytically inactive protein (PRIP), which was firstly identified in our laboratory, in the regulation of the function of protein kinases and phosphatases, especially in the phospho-dependent modulation of exocytosis. We found that PRIP directly interacted with the catalytic subunits of two distinct phosphatases (PP1 and PP2A) in a mutually exclusive manner and the interactions are regulated by phosphorylation of PRIP itself. Moreover,phospho-SNAP-25 (synaptosome-associated protein of 25kDa), a component of SNARE complex, was dephosphorylated by protein phosphatase-1, whose activity was regulated by PRIP. Noradrenalin secretionin PC12 cells was also modulated b y PRI P, which was correlated with the phospho-states of SNAP-25.Collectively, these results suggest that PRIP is involved in the regulation of the phospho-states of SNAP-25 by modulating the activity of PP1, thus regulating exocytosis.
|
-
-
-
[Presentation] Roles of PRIP in phospho-regulation of exocytosis through the interaction with protein phosphatases2012
Author(s)
Gao, J. Takeuchi, H., Zhang, Z., Sugiyama,G., Nagano, K. and Hirata, M.
Organizer
The 7th Korea-Japan Conference on Cellular Signaling for Young Scientist
Place of Presentation
Ulsan, Korea
Year and Date
20120217-0218
-
[Presentation] Phospho-dependent modulation of exocytosis by PRIP.2011
Author(s)
Gao, J., Takeuchi, H., Zhang, Z., Sugiyama, G., Nagano, K. and Hirata, M.
Organizer
The 10th JBS Biofrontier Symposium on New Aspects of Phospholipid Biology and Medicine 2011
Place of Presentation
Fukuoka, Japan
Year and Date
20111114-1116
-
[Presentation] Phospho-regulation of exocytosis by PRIP2011
Author(s)
akeuchi, H., Gao, J., Zhang, Z., Sugiyama, G., Nagano, K. and Hirata, M.
Organizer
The 10th JBS Biofrontier Symposium on New Aspects of Phospholipid Biology and Medicine 2011
Place of Presentation
Fukuoka, Japan
Year and Date
20111114-1116
-