Involvement of a novel molecule in the phospho-regulation of exocytosis using a cell-based model

2012 Fiscal Year Final Research Report

• Project/Area Number: 23792127
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Kyushu University
• Principal Investigator: GAO Jing 九州大学, 歯学研究院, 助教 (40585882)
• Project Period (FY): 2011 – 2012
• Keywords: 開口分泌 / リン酸化 / SNARE / ホスファターゼ

Research Abstract

In the current study, we investigated the involvement of phospholipase C-related but catalytically inactive protein (PRIP), which was firstly identified in our laboratory, in the regulation of the function of protein kinases and phosphatases, especially in the phospho-dependent modulation of exocytosis. We found that PRIP directly interacted with the catalytic subunits of two distinct phosphatases (PP1 and PP2A) in a mutually exclusive manner and the interactions are regulated by phosphorylation of PRIP itself. Moreover,phospho-SNAP-25 (synaptosome-associated protein of 25kDa), a component of SNARE complex, was dephosphorylated by protein phosphatase-1, whose activity was regulated by PRIP. Noradrenalin secretionin PC12 cells was also modulated b y PRI P, which was correlated with the phospho-states of SNAP-25.Collectively, these results suggest that PRIP is involved in the regulation of the phospho-states of SNAP-25 by modulating the activity of PP1, thus regulating exocytosis.

Research Products

• [Presentation] SNAP-25 phosphorylation causes down-regulation of SNARE complex formation. (2012)
  • Author(s): Gao, J., Takeuchi, H., Zhang, Z., Wang, DG. and Hirata, M.
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡市
  • Year and Date: 20121200
• [Presentation] Role of SNAP-25 phosphorylation by protein kinase A in SNARE complex formation. (2012)
  • Author(s): Gao, J., Takeuchi, H., Zhang, Z., Wang, D G. and Hirata, M.
  • Organizer: The 22ndIUBMB & 37th FEBS Congress
  • Place of Presentation: Sevilla, Spain
  • Year and Date: 20120904-0909
• [Presentation] Roles of PRIP in phospho-regulation of exocytosis through the interaction with protein phosphatases (2012)
  • Author(s): Gao, J. Takeuchi, H., Zhang, Z., Sugiyama,G., Nagano, K. and Hirata, M.
  • Organizer: The 7th Korea-Japan Conference on Cellular Signaling for Young Scientist
  • Place of Presentation: Ulsan, Korea
  • Year and Date: 20120217-0218
• [Presentation] Phospho-dependent modulation of exocytosis by PRIP. (2011)
  • Author(s): Gao, J., Takeuchi, H., Zhang, Z., Sugiyama, G., Nagano, K. and Hirata, M.
  • Organizer: The 10th JBS Biofrontier Symposium on New Aspects of Phospholipid Biology and Medicine 2011
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 20111114-1116
• [Presentation] Phospho-regulation of exocytosis by PRIP (2011)
  • Author(s): akeuchi, H., Gao, J., Zhang, Z., Sugiyama, G., Nagano, K. and Hirata, M.
  • Organizer: The 10th JBS Biofrontier Symposium on New Aspects of Phospholipid Biology and Medicine 2011
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 20111114-1116
• [Presentation] Involvement of PRIP in exocytosis through phospho-dependent regulation of SNAP-25. (2011)
  • Author(s): Gao, J., Takeuchi, H., Zhang, Z., Sugiyama, G. and Hirata, M.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市
  • Year and Date: 20110900