Study of inflammatory regulation by biotin via holocarboxylase synthetase

2012 Fiscal Year Final Research Report

• Project/Area Number: 23792140
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Tohoku University
• Principal Investigator: KUROISHI Toshinobu 東北大学, 大学院・歯学研究科, 助教 (30400261)
• Project Period (FY): 2011 – 2012
• Keywords: 免疫 / 炎症 / 金属アレルギー / ビオチン

Research Abstract

Biotin is a water-soluble B complex vitamin found in all organisms. To elucidate the mechanisms of inflammatory regulation by biotin, a holocarboxylase synthetase knock-down macrophage (HCS-KD cells) was constructed. The holocarboxylase synthetase (HCS) is a key-enzyme in cellular biotin metabolisms. The production of tumor necrosis factor-α, an important bioactive protein that involved with inflammation, was decreased in the HCS-KD cells. On the other hand, CD11b, an adhesive protein that involved with inflammation, was up-regulated in the HCS-KD cells.

Research Products

• [Journal Article] Analgesic effects of the non-nitrogen- containing bisphosphonates etid- ronate and clodronate, independent of anti-resorptive effects on bone (2013)
  • Author(s): Kim, Y., Seiryu, M., Okada, S., Kuroishi, T., Takano-Yamamoto, T., Sugawara, S., Endo, Y
  • Journal Title: European Journal of Pharmacology Volume: 699 Pages: 14-22
  • Peer Reviewed
• [Journal Article] In vitro cytotoxicity of zoledronate (nitrogen-containing bisphosphonate: NBP)and/or etidronate (non-NBP) in tumour cells and periodontal cells (2012)
  • Author(s): Tanaka, Y., Nagai, Y., Dohdoh, M., Oizumi, T., Ohki, A., Kuroishi, T., Sugawara, S., Endo, Y
  • Journal Title: Archives of Oral Biology
  • DOI: doi:pii:S0003-9969(12)00408-6.10.1016/j.arc horalbio.2012.11.010.
  • Peer Reviewed
• [Journal Article] Histamine reduces susceptibility to natural killer cells via down- regulation of NKG2D ligands on human monocytic leukaemia THP-1 cells (2012)
  • Author(s): Nagai, Y., Tanaka, Y., Kuroishi, T., Sato, R., Endo, Y., Sugawara, S
  • Journal Title: Immunology Volume: 136 Pages: 103-114
  • Peer Reviewed
• [Journal Article] Biotin (2012)
  • Author(s): Zempleni, J., Kuroishi, T.
  • Journal Title: Advance in Nutrition Volume: 3 Pages: 213-214
  • Peer Reviewed
• [Journal Article] Biotin requirements for DNA damage prevention (2012)
  • Author(s): Zempleni, J., Teixeira, D.C., Kuroishi, T., Cordonier, E.L., Baier, S.
  • Journal Title: Mutation Research Volume: 733 Pages: 58-60
  • Peer Reviewed
• [Journal Article] Stimulation of Ly-6G on neutrophils in LPS-primed mice induces platelet-activating factor (PAF)-mediated anaphylaxis- like shock (2011)
  • Author(s): Tanaka, Y., Nagai, Y., Kuroishi, T., Endo, Y., Sugawara, S.
  • Journal Title: Journal of Leukocyte Biology Volume: 91 Pages: 485-494
  • Peer Reviewed
• [Journal Article] Biotinylation is a natural, albeit rare, modification of human histones (2011)
  • Author(s): Kuroishi, T., Rios-Avila, L., Pestinger, V., Wijeratne, S.S.K., Zempleni, J
  • Journal Title: Molecular Genetics and Metabolism Volume: 104 Pages: 537-545
  • Peer Reviewed
• [Presentation] IL-1β augments nickel-induced nitric oxide production by mouse dermal fibroblast (2012)
  • Author(s): Kuroishi, T., Endo, Y., Sugawara, S.
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20121205-07
• [Presentation] ニッケル刺激マウス線維芽細胞によるNO産生とIL-1βによるその増強 (2012)
  • Author(s): 黒石智誠,遠藤康男,菅原俊二
  • Organizer: 第54回歯科基礎医学会
  • Place of Presentation: 郡山市
  • Year and Date: 20120915-16
• [Book] Present Knowledge inNutrition, 10th edition (2012)
  • Author(s): Zempleni, J., Wijeratne, S.S.K., Kuroishi, T
  • Total Pages: 359-374
  • Publisher: Wiley-Blackwell