Development of the effective bone augmentation using intracellular transfection of anti-apoptosis protein HSP27

2013 Fiscal Year Final Research Report

• Project/Area Number: 23792215
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Prosthetic dentistry
• Research Institution: Niigata University
• Principal Investigator: KAWASAKI Maiko 新潟大学, 医歯学総合病院, 助教 (40584587)
• Project Period (FY): 2011 – 2013
• Keywords: HSP27 / 骨芽細胞 / アポトーシス / トランスフェクション法 / 骨増成

Research Abstract

In the present study, to explore a new way of cell transplantation for bone augmentation, we evaluate the application of HSP27, an anti-apoptotic protein to the transplanted cell. The over expression of HSP27 did not affect the cell proliferation and mineralization ability of osteoblast, however, apoptosis was diminished by the over expression of HSP27. Furthermore, transplanted cell apoptosis was also inhibited and proliferation marker expressing cells were increased at the initial stage of cell transplantation.

Research Products

• [Journal Article] R-spondins/Lgrs expression in tooth development (2014)
  • Author(s): Kawasaki M, Porntaveetus T, Kawasaki K, Oommen S, Otsuka-Tanaka Y, Hishinuma M, Nomoto T, Maeda T, Takubo K, Suda T, Sharpe PT, Ohazama A
  • Journal Title: Dev Dyn Volume: (Epub ahead of print)
  • DOI: 10.1002/dvdy.24124
  • Peer Reviewed
• [Journal Article] Adult Human Gingival Epithelial Cells as a Source for Whole-tooth Bioengineering (2013)
  • Author(s): Angelova Volponi A, Kawasaki M, Sharpe PT
  • Journal Title: J Dent Res Volume: 92(4) Pages: 329-334
  • DOI: 10.1177/0022034513481041
  • Peer Reviewed
• [Journal Article] Multiple postnatal craniofacial anomalies are characterized by conditional loss of polycystic kidney disease 2 (Pkd2) (2013)
  • Author(s): Khonsari RH, Ohazama A, Raouf R, Kawasaki M, Kawasaki K, Porntaveetus T, Ghafoor S, Hammond P, Suttie M, Odri GA, Sandford RN, Wood JN, Sharpe PT
  • Journal Title: Hum Mol Genet Volume: 22(9) Pages: 1873-1885
  • DOI: 10.1093/hmg/ddt041
  • Peer Reviewed
• [Journal Article] The buccohypophyseal canal is an ancestral vertebrate trait maintained by modulation in sonic hedgehog signaling (2013)
  • Author(s): Khonsari RH, Seppala M, Pradel A, Dutel H, Clément G, Lebedev O, Ghafoor S, Rothova M, Tucker A, Maisey JG, Fan CM, Kawasaki M, Ohazama A, Tafforeau P, Franco B, Helms J, Haycraft CJ, David A, Janvier P, Cobourne MT, Sharpe PT
  • Journal Title: BMC Biol Volume: 11(1) Pages: 27
  • Peer Reviewed
• [Journal Article] Oral lining mucosa development depends on mesenchymal microRNAs (2013)
  • Author(s): Otsuka-Tanaka Y, Oommen S, Kawasaki M, Kawasaki K, Imam N, Jalani-Ghazani F, Hindges R, Sharpe PT, Ohazama A
  • Journal Title: J Dent Res Volume: 92(3) Pages: 229-234
  • DOI: 10.1177/0022034512470830
  • Peer Reviewed
• [Journal Article] Cytoplasmic plaque formation in hemidesmosome development is dependent on SoxF transcription factor function (2012)
  • Author(s): Oommen S, Francois M, Kawasaki M, Murrell M, Kawasaki K, Porntaveetus T, Ghafoor S, Young NJ, Okamatsu Y, McGrath J, Koopman P, Sharpe PT, Ohazama A
  • Journal Title: PLoS One Volume: 7(9) Pages: e43857
  • DOI: 10.1371/journal.pone.0043857
  • Peer Reviewed
• [Journal Article] Distinct roles of microRNAs in epithelium and mesenchyme during tooth development (2012)
  • Author(s): Oommen S, Otsuka-Tanaka Y, Imam N, Kawasaki M, Kawasaki K, Jalani-Ghazani F, Anderegg A, Awatramani R, Hindges R, Sharpe PT, Ohazama A
  • Journal Title: Dev Dyn Volume: 241(9) Pages: 1465-1472
  • DOI: 10.1002/dvdy.23828