2013 Fiscal Year Final Research Report
Relationship between metastasis of cancer and abnormality of tumor endothelial cell
| Project/Area Number |
23792306
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
NORITAKA Ohga 北海道大学, 歯学研究科(研究院), 助教 (40548202)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 腫瘍血管新生 / 転移 / 多様性 / 薬剤抵抗性 / 幹細胞性 |
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| Research Abstract |
It is an important concept of tumor angiogenesis that tumor endothelial cells (TEC) are genetically normal and homogeneous. However, we had previously reported that TEC differ from normal endothelial cells (NEC). Whether the characteristics of TEC derived from different tumors differ remains unknown. To elucidate this, we isolated two types of TEC from highly metastatic tumor and low metastatic tumor and compared their characteristics. High metastatic tumor-derived TEC (HM-TEC) showed higher proliferative activity and invasive activity than low metastatic tumor-derived TEC (LM-TEC). Moreover, the mRNA expression levels of proangiogenic genes, such as VEGF-R1 and 2, VEGF and hypoxia (HIF-1) were higher in HM-TEC compared with those in LM-TEC. Furthermore, HM-TEC showed higher mRNA expression levels of the stemness-related genes compared with LM-TEC. These results indicate that TEC from high metastatic tumors have developed more abnormality than those from low metastatic tumors.
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Research Products
(10 results)
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[Journal Article] Heterogeneity of Tumor Endothelial Cells : Comparison between Tumor Endothelial Cells Isolated from Highly Metastatic and Low Metastatic Tumors2012
Author(s)
Ohga N., Ishikawa S., Maishi N., Akiyama K., Hida Y.,Kawamoto T., Sadamoto Y., Osawa T.,Yamamoto K., Kondoh M., Ohmura H., Shinohara N., Nonomura K., Shindoh M., Hida K.
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Journal Title
Am J Pathol
Volume: 180(3)
Pages: 1294-1307
Peer Reviewed
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[Journal Article] Tumor endothelial cells acquire drug resistance by MDR1 upregulation via VEGF signaling in tumor microenvironment2012
Author(s)
Akiyama K., Ohga N., Hida Y., Kawamoto T., Sadamoto Y., Ishikawa S., Maishi N., Akino T., Kondoh M., Matsuda A., Inoue N., Shindoh M. and Hida K.
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Journal Title
Am J Pathol
Volume: 180(3)
Pages: 1283-1293
Peer Reviewed
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[Presentation] Heterogeneity of Tumor Endothelial Cells : Comparison between Tumor Endothelial Cells Isolated from Highly Metastatic and Low Metastatic Tumors2011
Author(s)
Ohga N., Ishikawa S., Maishi N., Akiyama K., Hida Y., Kawamoto T., Sadamoto Y., Kondoh M., Ohsawa T., Yamamoto K., Ohmura H., Shindoh M., Hida K.
Organizer
The 19th Annual Meeting of the Japanese Vascular Biology and Medicine Organization
Place of Presentation
東京ステーションコンファレンス(Tokyo, Japan)
Year and Date
20111208-10
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[Presentation] Comparative characterization of tumor endothelial cells isolated from highly and low metastatic tumors2011
Author(s)
Ishikawa S., Hida Y., Akiyama K., Kitayama K., Kondoh M., Maishi N., Kawamoto T., Ohsawa T., Yamamoto K., Shindoh M., Hida K.
Organizer
The 5^<th> Meeting of Asian Society of Oral and Maxillofacial Pathology,"A New Insights into Tumor endothelial cell"
Place of Presentation
九州大学医学部百年講堂(Fukuoka, Japan)
Year and Date
2011-08-24
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