A study on epithelial-mesenchymal transition mediated by deltaNp63 in invasion and metastasis of oral squamous cell carcinoma

2013 Fiscal Year Final Research Report

• Project/Area Number: 23792358
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kyushu University
• Principal Investigator: KAWANO Shintaro 九州大学, 大学病院, 講師 (00398067)
• Project Period (FY): 2011 – 2013
• Keywords: 上皮間葉転換 / 口腔扁平上皮癌 / deltaNp63 / 浸潤 / 転移

Research Abstract

In this study, we investigated the role of deltaNp63 in EMT during progression of oral squamous cell carcinoma (OSCC). The expressions of deltaNp63 and epithelial markers was detected in the OSCC cells, but not in SQUU-B cells (high metastatic potential). Mesenchymal markers were strongly expressed in the SQUU-B cells. Knockdown of endogenous deltaNp63 in HSC-2 cells induced morphological changes to the spindle shape, decreased the expression of epithelial markers, increased the expression of mesenchymal markers, increased migration. By contrast, SQUU-B cells overexpressing deltaNp63 showed changed their morphology from stromal cell-like to epithelial cells. Immunohistochemical staining revealed decreased intensity of deltaNp63 was positively associated with metastases and poor prognosis in the OSCC patients. These results indicated that deltaNp63 downregulation in cancer cells induces a mesenchymal phenotype that is related to tumor progression of OSCC.

Research Products

• [Journal Article] Possible involvement ofΔNp63 downregulation in the invasion and metastasis of oral squamous cell carcinoma via induction of a mesenchymal phenotype (2014)
  • Author(s): Y. Goto, S. Kawano, R. Matsubara, T. Kiyosue, M. Hirano, T. Jinno, Y. Maruse, T. Toyoshima, R. Kitamura, H. Tanaka, K. Oobu, S. Nakamura
  • Journal Title: Clinical & Experimental Metastasis Volume: 214 Pages: 289-306
• [Journal Article] IncreasedΔNp63 expression is predictive of malignant transformation in oral epithelial dysplasia and poor prognosis in oral squamous cell carcinoma (2011)
  • Author(s): R. Matsubara, S. Kawano, T. Kiyosue, Y. Goto, M. Hirano, T. Toyoshima, R. Kitamura, K. Oobu, and S. Nakamura
  • Journal Title: International Journal of Oncology Volume: 39 Pages: 1391-1399
• [Presentation] 口腔扁平上皮癌におけるIL-6の発現と臨床病理学的所見との関連 (2013)
  • Author(s): 神野哲平, 川野真太郎, 松原良太, 清末崇裕, 後藤雄一, 平野充広, 田中秀明, 丸瀬靖之, 森山雅文, 豊嶋健史, 中村誠司
  • Organizer: 第37回日本頭頸部癌学会
  • Year and Date: 2013-06-13
• [Presentation] 口腔扁平上皮癌高転移株より分離した上皮-間葉転換細胞の特性 (2013)
  • Author(s): 平野充広, 川野真太郎, 松原良太, 清末崇裕, 後藤雄一, 神野哲平, 丸瀬靖之, 豊嶋健史, 北村亮二, 田中秀明, 大部一成, 中村誠司
  • Organizer: 第17回九州地区口腔癌研究会学術講演会
  • Year and Date: 2013-06-07
• [Presentation] 口腔扁平上皮癌におけるinterleukin-6発現と臨床病理学的所見との関連 (2013)
  • Author(s): 神野哲平, 川野真太郎, 平野充広, 松原良太, 清末崇裕, 後藤雄一, 丸瀬靖之, 今藤彰子, 豊嶋健史, 田中秀明, 大部一成, 中村誠司
  • Organizer: 第31回日本口腔腫瘍学会総会
  • Year and Date: 2013-01-24
• [Presentation] 口腔扁平上皮癌の浸潤・転移におけるΔNp63を介した上皮-間葉転換の関与 (2011)
  • Author(s): 後藤雄一, 川野真太郎, 松原良太, 清末崇裕, 平野充広, 神野哲平, 豊嶋健史, 北村亮二, 大部一成, 中村誠司
  • Organizer: 第56回日本口腔外科学会総会
  • Year and Date: 2011-10-24
• [Presentation] 口腔扁平上皮癌における浸潤・転移の分子機構の解明-ΔNp63の発現と上皮-間葉転換との関連について- (2011)
  • Author(s): 後藤雄一, 川野真太郎, 松原良太, 清末崇裕, 平野充広, 豊嶋健史, 北村亮二, 大部一成, 中村誠司
  • Organizer: 第65回日本口腔科学会学術集会
  • Year and Date: 2011-04-21
• [Remarks]
  • URL: http://www.omfs1.dent.kyushu-u.ac.jp