Development of specific molecular target therapy for intractable disease in the oral and maxillofacial Region using the nanobubble and ultrasound

2012 Fiscal Year Final Research Report

• Project/Area Number: 23792377
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: IWANAGA Kenjiro 九州歯科大学, 歯学部, 助教 (20448484)
• Project Period (FY): 2011 – 2012
• Keywords: ソノポレーション / ナノバブル / 遺伝子導入

Research Abstract

Polyethylene glycosylated (PEG) liposome avoids uptake by the reticulo-endothelial system, thus improving drug delivery to tumor while decreasing systemic toxicity. PEGliposomes containing doxorubicin, Doxil, have been used to treat Kaposi's sarcoma and ovarian cancer in clinics. Doxil(R) circulates around the body at a stable state and accumulates in tumor by passive targeting. Although it is difficult to release doxorubicin after accumulation into the tumor, we made new bubble liposomes from Doxi(R) and investigated the effect on cytotoxicity in human gingival squamous cell carcinoma Ca9-22 cells by combination with this new Doxil(R) bubble liposome,encapsulated doxorubicin bubble liposomes (EDBL), and ultrasound. The combination of EDBL and ultrasound was found to enhance the cytotoxity of Ca9-22 cells treated by doxorubicin. This findings suggest that combination of EDBL and ultrasound might be an effective drug delivery system against cancer cells.

Research Products

• [Journal Article] Mechanisms of G1 cell cycle arrest and apoptosis in myeloma cells induced by hybrid-compound histone deacetylase inhibitor (2013)
  • Author(s): Fujii, S., Okinaga, T., Ariyoshi, W., Takahashi, O., Iwanaga, K., Nishino, N., Tominaga, K., Nishihara, T
  • Journal Title: iochem Biophys Res Commun Volume: 434 Pages: 413-20
• [Journal Article] Ariyoshi,W.,Tominaga,K. Nishino,N and Nishihara,T:Novelhistone deacetylase inhibitor exhibits antitumor activity via apoptosis induction in oral squamous cell carcinoma (2011)
  • Author(s): Takahashi,O.,Okinaga,T.,Iwanaga,K., Habu,M
  • Journal Title: Journal of Biophysical Chemistry Volume: 2 Pages: 215-221
• [Journal Article] 2011. Inhibition of adjuvant arthritis in rats (2011)
  • Author(s): Sukedai,M., Ariyoshi, W., Okinaga, T, K. Iwanaga, M. Habu, I. Yoshioka, K. Tominaga, T. Nishihara
  • Journal Title: electroporation with interleukin-1 receptor antagonist. J. Interferon and Cytokine Res Volume: 31 Pages: 839-46
• [Presentation] 冨永和宏.ナノ・マイクロバブルと超音波を利用したドラッグデリバリーシステムの確立 (2013)
  • Author(s): 岩永賢二郎,沖永敏則,山本雅史,西原達次
  • Organizer: 第73回九州歯科学会総会
  • Place of Presentation: 北九州
  • Year and Date: 2013-05-19
• [Presentation] ナノバブルを利用した超音波遺伝子・ドラッグデリバリーシステムの確立 (2012)
  • Author(s): 岩永賢二郎
  • Organizer: 第72回九州歯科学会総会
  • Place of Presentation: 北九州
  • Year and Date: 2012-05-18