2012 Fiscal Year Final Research Report
Expression and function of RANKL in gingival epithelialum
| Project/Area Number |
23792487
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontal dentistry
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| Research Institution | Kyushu Dental College (2012)
Showa University (2011) |
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Principal Investigator |
USUI Michihiko 九州歯科大学, 歯学部・歯周病学分野, 准教授 (10453630)
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| Co-Investigator(Renkei-kenkyūsha) |
SATO Tsuyoshi 埼玉医科大学, 歯学部, 講師 (60406494)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 歯肉上皮細胞 / RANKL / PKA signaling / 破骨細胞形成 |
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| Research Abstract |
Periodontitis is a chronic inflammatory disease characterized by the destruction of alveolar bone. Receptor activator of nuclear factor kappa B ligand (RANKL) is known to be an essential factor for osteoclastogenesis. Recent clinical studies indicate that levels of RANKL in the gingival crevicular fluid are increased in periodontitis patients. Although the gingival sulcus is liend by gingival epithelial cells (GECs), RANKL expression in GECs is not fully understood. RT-PCR, western blotting and immunohistochemistry were performed to confirm RANKL expression in GECs and in gingival tissue. Ca9-22 cells, a human gingival epithelial cell line, were treated with tumor necrosis factor (TNF)-α, antibodies against TNF receptors, and an inhibitor and an activator of PKA signaling. The levels of RANKL expression were examined by real time PCR and western blotting. GECs and bone marrow-derived osteoclast precursors were co-cultured to examine the effect of GECs-produced RANKLon osteoclast formation. RANKL mRNA and protein were expressed in GECs. Immunohistochemistry also showed RANKL and TNF-α expression in gingival epithelium, but not in gingival connective tissue. TNF-α increased the levels of RANKL expression in GECs. TNF-α-induced RANKL expression was inhibited by antibody against TNF receptor type 1 (TNFR1) and an inhibitor of PKA signaling. GECs constitutively induced osteoclast formation. This effect was increased significantly by TNF-α and activator of PKA signaling, and prevented by RANK-Fc.TNF-α induces RANKL expression through PKA signaling in GECs, which regulate osteoclastogenesis. GECs may take part in progression of alveolar bone destruction by periodontitis.
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Research Products
(13 results)
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[Journal Article] 超音波照射のヒト歯肉上皮細胞に対する影響について2011
Author(s)
臼井通彦, 滝口尚, 史春, Enkhzaya Guruudivaa, 宮澤康, 菅野真莉加, 野瀬冬樹, 斎藤彰大, 菊池真理子, 冨永和宏, 西原達次, 根岸洋一,山本松男
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Journal Title
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[Presentation] Hypothalamic-pituitary-adrenal axis is essential for regulation of both bone and fat metabolism via melanocortin receptor2012
Author(s)
Sato T, Chida D, Iwata T, Usui M, Enoki Y, Matsumoto M, Xu R, Sunamura S, Ochi H, Fukuda T, Takeda S, Yoda T
Organizer
American Society of Bone and Mineral Research 34th Annual Meeting
Place of Presentation
Minneapolis, USA
Year and Date
20121000
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