The effects of the degradation of mucin by Streptococci on the mechanism of oral malodor

2013 Fiscal Year Final Research Report

• Project/Area Number: 23792532
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: Fukuoka Dental College
• Principal Investigator: TANIGUCHI NAO 福岡歯科大学, 歯学部, 講師 (60372885)
• Project Period (FY): 2011 – 2013
• Keywords: 揮発性硫黄化合物 / 口腔内細菌叢 / 口臭 / 高速シーケンス解析 / グルコシダーゼ / 唾液 / β-galactosidase

Research Abstract

Salivary beta-galactosidase activity from the patients complaining of halitosis was measured and compared between the periodontally healthy group and the periodontitis group. Positive correlations were observed between the beta-galactosidase activity and oral malodor levels in the periodontally healthy group. Concerning the clinical parameter, the plaque index and tongue coating score were positively correlated with beta-galactosidase activity in the periodontal healthy group. Next, the bacterial compositions of saliva exhibiting higher and lower malodor were explored in order to determine the specific microbial community related to oral malodor. In addition, the compositions of oral microbiota associated with high hydrogen sulfide and methyl mercaptan production were determined. Tongue coating is main sources of oral malodor, and therefore the beta-galactosidase activity of tongue coating was measured.

Research Products

• [Journal Article] Effects of S-PRG eluate on oral biofilm and oral malodor (2014)
  • Author(s): Suzuki N, Yoneda M, Haruna K, Masuo Y, Nishihara T, Nakanishi K, Yamada K, Fujimoto A, Hirofuji T
  • Journal Title: Archives of Oral Biology Volume: 59(4) Pages: 407-413
  • DOI: 10.1016/j.oooo.2013.12.400
• [Journal Article] Lactobacillus salivarius WB21-containing tablets for the treatment of oral malodor : a double blind, randomized, placebo-controlled crossover trial (2014)
  • Author(s): Suzuki N, Yoneda M, Tanabe K, Fujimoto A, Iha K, Seno K, Yamada K, Iwamoto T, Masuo Y, Hirofuji T
  • Journal Title: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology Volume: 117(4) Pages: 462-470
  • DOI: 10.1016/j.oooo.2013.12.400
• [Journal Article] Supervised machine learning-based prediction of oral malodor based on the microbiota in saliva samples (2014)
  • Author(s): Nakano Y, Takeshita T, Kamio N, Shiota S, Shibata Y, Suzuki N, Yoneda M, Hirofuji T, Yamashita Y
  • Journal Title: Artificial Intelligence in Medicine Volume: 60(2014) Pages: 97-101
  • DOI: 10.1016/j.artmed.2013.12.001
• [Journal Article] Effect of mouth cleaning with hinokitiol-containing gel on oral malodor : a randomized, open-label pilot study (2013)
  • Author(s): Iha K, Suzuki N, Yoneda M, Takeshita T, Hirofuji T
  • Journal Title: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology Volume: 116(4) Pages: 433-439
  • DOI: 10.1016/j.oooo.2013.05.021
• [Journal Article] Mix red-complex bacterial infection in periodontitis (2013)
  • Author(s): Suzuki N, Yoneda M, Hirofuji T
  • Journal Title: International Journal of Dentistry. 2013 Volume: 587279
  • DOI: 10.1155/2013/587279
• [Journal Article] Awareness among dental hygienists with regard to treatment options for halitosis (2012)
  • Author(s): Yoneda M, Kashio N, Uchida H, Suzuki N, Matsuura H, Naito T, Koga C, Yasukochi H, Matsuura M, Hirofuji T
  • Journal Title: International Journal of Clinical Preventive Dentistry Volume: 8(3) Pages: 173-179
• [Journal Article] Effect of S-PRG eluate on biofilm formation and enzyme activity of oral bacteria (2012)
  • Author(s): Yoneda M, Suzuki N, Masuo Y, Fujimoto A, Iha K, Yamada K, Iwamoto T, Hirofuji T
  • Journal Title: International Journal of Dentistry Volume: ID814913 Pages: 6
  • DOI: 10.1155/2012/814913
• [Journal Article] Effects of oil drops containing Lactobacillus salivarius WB21 on periodontal health and oral microbiota producing volatile sulfur compounds (2012)
  • Author(s): Suzuki N, Tanabe K, Takeshita T, Yoneda M, Iwamoto T, Oshiro S, Yamashita Y, Hirofuji T
  • Journal Title: Journal Breath Research Volume: 6(1) Pages: 017106
  • DOI: 10.1088/1752-7155/6/1/017106
• [Journal Article] Discrimination of the oral microbiota associated with high hydrogen sulfide and methyl mercaptan production (2012)
  • Author(s): Takeshita T, Suzuki N, Nakano Y, Yasui M, Yoneda M, Shimazaki Y, Hirofuji T, Yamashita Y
  • Journal Title: Scientific Reports Volume: 2 Pages: 215
  • DOI: 10.1038/srep00215
• [Journal Article] Salivaryβ-galactosidase activity affects physiological oral malodor (2012)
  • Author(s): Masuo Y, Suzuki N, Yoneda M, Naito T, Hirofuji T
  • Journal Title: Archives of Oral Biology Volume: 57(1) Pages: 87-93
  • DOI: 10.1016/j.archoralbio.2011.07.015
• [Journal Article] The variable etiology of oral pathologic halitosis : a case series (2012)
  • Author(s): Y oneda M, Suzuki N, Mocedo SM, Fujimoto A, Iha K, Koga C, Matsuura M, Hirofuji T
  • Journal Title: Smile Dental Journal Volume: 7(3) Pages: 50-56
• [Journal Article] Confusing Endodontic cases : case series report (2011)
  • Author(s): Yoneda M, Suzuki N, Macedo SM, Anan H, Hirofuji T
  • Journal Title: Smile Dental Journal Volume: 6(2) Pages: 26-31
• [Journal Article] Enterococcus faecium WB2000 inhibits Biofilm Formation by Oral Cariogenic Streptococci (2011)
  • Author(s): Suzuki N, Yoneda M, Hatano Y, Iwamoto T, Masuo Y, and Hirofuji T
  • Journal Title: International Journal of Dentistry 2011 Volume: 834151 Pages: 5
  • DOI: 10.1155/2011/834151
• [Presentation] プロバイオティクスを用いた口腔疾患の予防管理への取り組み (2013)
  • Author(s): 岩元知之、鈴木奈央、米田雅裕、山田和彦、桝尾陽介、藤本暁江、伊波幸作、瀬野恵衣、廣藤卓雄
  • Organizer: 歯科人間工学会
  • Place of Presentation: 名古屋市
  • Year and Date: 20131122-23
• [Presentation] S-PRG溶出液が口腔バイオフィルムと口臭に及ぼす阻害作用 (2013)
  • Author(s): 春名一人、鈴木奈央、米田雅裕、山田潤一、桝尾陽介、西原哲世、仲西宏介、山田和彦、岩元知之、藤本暁江、伊波幸作、瀬野恵衣、安忠大、入江丹佳子、廣藤卓雄
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 秋田市
  • Year and Date: 20131017-18
• [Presentation] Improvement of oral malodor using Lactobacillus salivarius WB21-containing tablets (2013)
  • Author(s): Suzuki N, Yoneda M, Tanabe K, Fujimoto A, Iha K, Seno K, Iwamoto T, Yamada K, Masuo Y, Haruna K, and Hirofuji T
  • Organizer: WCPD
  • Place of Presentation: Budapest
  • Year and Date: 20131009-12
• [Presentation] ヒノキチオール配合ジェルを用いた口腔ケアが口臭及び口腔内環境に与える影響 (2013)
  • Author(s): 伊波幸作、鈴木奈央、米田雅裕、山田和彦、岩元知之、桝尾陽介、藤本暁江、瀬野恵衣、春名一人、安忠大、入江丹佳子、西原哲世、仲西宏介、廣藤卓雄
  • Organizer: 第138回日本歯科保存学会春季学術大会
  • Place of Presentation: 福岡市
  • Year and Date: 20130627-28
• [Presentation] ヒト口腔内細菌叢の構成に対する亜鉛イオンの影響 (2012)
  • Author(s): 中野善夫、鈴木奈央、米田雅裕、廣藤卓雄、横瀬勝美、桑田文幸
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡市
  • Year and Date: 20121214-16
• [Presentation] S-PRG溶出液とS-PRG配合歯面研磨剤の口腔バイオフィルムへの影響 (2012)
  • Author(s): 桝尾陽介、鈴木奈央、米田雅裕、安忠大、仲西宏介、藤本暁江、伊波幸作、瀬野恵衣、山田和彦、岩元知之、廣藤卓雄
  • Organizer: 第137回日本歯科保存学会秋季学術大会
  • Place of Presentation: 広島市
  • Year and Date: 20121122-23
• [Presentation] ヒノキチオール配合口腔ケア用ジェルによる口腔清掃の口臭改善効果 (2012)
  • Author(s): 伊波幸作、鈴木奈央、藤本暁江、米田雅裕、山田和彦、岩元知之、桝尾陽介、西原哲世、金子元気、春名一人、廣藤卓雄
  • Organizer: 第137回日本歯科保存学会秋季学術大会
  • Place of Presentation: 広島市
  • Year and Date: 20121122-23
• [Presentation] 福岡歯科大学口腔医療センター口臭科の患者解析および口臭恐怖症の1症例 (2012)
  • Author(s): 米田雅裕、古賀千尋、鈴木奈央、内藤徹、廣藤卓雄
  • Organizer: 第27回日本歯科心身医学会総会・学術大会
  • Place of Presentation: 川越市
  • Year and Date: 20120901-02
• [Presentation] 真性口臭症におけるLactobacillus salivarius WB21株配合錠菓の有用性の検討 (2012)
  • Author(s): 藤本暁江、鈴木奈央、米田雅裕、伊波幸作、田邊一成、山田和彦、岩元知之、桝尾陽介、廣藤卓雄
  • Organizer: 第3回日本口臭学会
  • Place of Presentation: 東京都
  • Year and Date: 20120707-08
• [Presentation] 塩化亜鉛溶液の洗口による口腔内細菌叢の変動 (2012)
  • Author(s): 鈴木奈央、中野善夫、米田雅裕、横瀬勝美、桑田文幸、廣藤卓雄
  • Organizer: 第3回日本口臭学会
  • Place of Presentation: 東京都
  • Year and Date: 20120707-08
• [Presentation] 塩化亜鉛曝露による口腔細菌構成の変化 (2012)
  • Author(s): 鈴木奈央、中野善夫、畑野優子、米田雅裕、横瀬勝美、桑田文幸、廣藤卓雄
  • Organizer: 第136回日本歯科保存学会春季大会
  • Place of Presentation: 宜野湾市
  • Year and Date: 20120628-29
• [Presentation] Lactobacillus salivarius WB21株配合錠菓の口臭抑制効果についての臨床研究～ランダム化二重盲検プラセボ対照クロスオーバー試験～ (2012)
  • Author(s): 藤本暁江、鈴木奈央、米田雅裕、田邊一成、山田和彦、岩元知之、桝尾陽介、伊波幸作、廣藤卓雄
  • Organizer: 第136回日本歯科保存学会春季大会
  • Place of Presentation: 宜野湾市
  • Year and Date: 20120628-29
• [Presentation] S-PRGイオン溶出液とS-PRG配合歯面研磨剤の口腔バイオフィルムへの影響 (2012)
  • Author(s): 桝尾陽介、鈴木奈央、米田雅裕、安忠大、仲西宏介、藤本暁江、伊波幸作、瀬野恵衣、山田和彦、岩元知之、廣藤卓雄
  • Organizer: 第39回福岡歯科大学学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-11-18
• [Presentation] βガラクトシダーゼの局在に関する検討および酵素活性と口臭との関連性について (2011)
  • Author(s): 桝尾陽介、鈴木奈央、米田雅裕、内藤徹、山田和彦、岩元知之、藤本暁江、廣藤卓雄
  • Organizer: 第4回総合歯科協議会
  • Place of Presentation: 新潟市
  • Year and Date: 20111119-20
• [Presentation] S-PRG溶出液が歯周病原性細菌のプロテアーゼ活性および共凝集活性におよぼす影響 (2011)
  • Author(s): 桝尾陽介、米田雅裕、鈴木奈央、山田和彦、岩元知之、藤本暁江、伊波幸作、廣藤卓雄
  • Organizer: 第135回日本歯科保存学会秋季学術大会
  • Place of Presentation: 大阪市
  • Year and Date: 20111020-21
• [Presentation] 塩化亜鉛溶液を用いた洗口による口腔常在フローラの変化 (2011)
  • Author(s): 鈴木奈央、中野善夫、畑野優子、米田雅裕、横瀬勝美、桑田文幸、廣藤卓雄
  • Organizer: 第135回日本歯科保存学会秋季学術大会
  • Place of Presentation: 大阪市
  • Year and Date: 20111020-21
• [Presentation] 乳酸菌配合歯磨剤の口腔衛生改善効果についての臨床研究 (2011)
  • Author(s): 畑野優子、鈴木奈央、米田雅裕、山田和彦、岩元知之、桝尾陽介、藤本暁江、伊波幸作、廣藤卓雄
  • Organizer: 第135回日本歯科保存学会秋季学術大会
  • Place of Presentation: 大阪市
  • Year and Date: 20111020-21
• [Presentation] 硫化水素およびメチルメルカプタン高産生口腔常在フローラの細菌構成 (2011)
  • Author(s): 竹下徹、鈴木奈央、中野善夫、米田雅裕、廣藤卓雄、山下喜久
  • Organizer: 第53回歯科基礎医学会
  • Place of Presentation: 岐阜市
  • Year and Date: 20110930-1002
• [Presentation] Lactobacillus salivarius WB21配合オイルの口腔内投与による口腔内環境の改善効果についての介入研究 (2011)
  • Author(s): 鈴木奈央、田邊一成、竹下徹、米田雅裕、岩元知之、大城末子、山下喜久、廣藤卓雄
  • Organizer: 第134回日本歯科保存学会春季大会
  • Place of Presentation: 千葉市
  • Year and Date: 20110609-10
• [Presentation] Enterococcus faecium WB2000株のmutans streptococciバイオフィルム形成に対する阻害作用についてのin vitro実験 (2011)
  • Author(s): 畑野優子、鈴木奈央、米田雅裕、山田和彦、岩元知之、桝尾陽介、藤本暁江、伊波幸作、廣藤卓雄
  • Organizer: 第134回日本歯科保存学会春季大会
  • Place of Presentation: 千葉市
  • Year and Date: 20110609-10
• [Presentation] S-PRG溶出液が歯周病原性細菌のプロテアーゼ活性および共凝集活性におよぼす影響 (2011)
  • Author(s): 桝尾陽介、米田雅裕、鈴木奈央、畑野優子、岩元知之、廣藤卓雄
  • Organizer: 第54回日本歯周病学会秋季学術大会
  • Place of Presentation: 下関市
  • Year and Date: 2011-09-24
• [Book] Relationship between oral malodor and oral microbiota. Oral Health Care-rosthodontics, Periodontology, Biology, Research and Systemic Conditions (2012)
  • Author(s): Suzuki N, Yoneda M, Hirofuji T
  • Publisher: In Tech(ISBN 978-953-51-0040-9)