2012 Fiscal Year Final Research Report
Genome - wide analysis for PRC1 - bound RNA molecules .
| Project/Area Number |
23810035
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
System genome science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MASUI Osamu 独立行政法人理化学研究所, 免疫器官形成研究グループ, 研究員 (30579305)
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| Project Period (FY) |
2011 – 2012
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| Keywords | ポリコーム転写抑制複合体 / RNA / RIP |
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| Research Abstract |
Polycomb repressive complexes (PRC1 and PRC2) are important protein complexes, which carry out epigenetic gene silencing by adding post-translational modifications to Histone proteins. PRC1 and PRC2 have been proposed to execute their transcriptional silencing function through binding with RNA molecules. Using RNA immunoprecipitation combined with next-generation DNA sequencer (RIP-seq) against Ring1B protein, a component of PRC1, we have recently identified those RNA molecules in a genome-wide manner. Surprisingly, transcripts of many components of PRC1 are included in the Ring1B-bound RNA fractions. These results suggest that the amount of PRC components may be controlled by PRCs themselves, providing a fine-tuning mechanism to maintain constant expression level of PRCs.
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