2012 Fiscal Year Final Research Report
Analysis of function ofhuman epidermal growth factor receptor using yeast
| Project/Area Number |
23860074
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
FUKUDA NOBUO 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究員 (00613548)
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| Project Period (FY) |
2011 – 2012
|
| Keywords | 酵母フェロモン応答 / ヒト受容体 / 自己リン酸化 / 膜局在性 |
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| Outline of Final Research Achievements |
In this study, we established a methodology to analyze function of human epidermal growth factor receptor (EGFR) by using yeast signal transduction as readout. EGFR is activated by autophosphorylation following dimerization. We succeeded in restoration of yeast signal transduction based on molecular interaction between constitutively activated EGFR and its adapter that recognizes and binds to the phosphorylated residue of EGFR. Our approach will provide a simple and easy tool for evaluation of EGFR inhibitors which are potential seed compounds for anticancer drugs.
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| Free Research Field |
工学
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