2012 Fiscal Year Final Research Report
Coherence of directional sensing, polarity, and motility in collective cell migration
| Project/Area Number |
23870006
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAKAJIMA Akihiko 東京大学, 大学院・総合文化研究科, 特任研究員 (90612119)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 細胞運動 / 粘菌 / 走化性 / 適応 / 自己組織化 / 反応拡散波 / 振動 |
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| Research Abstract |
Chemotaxis is one of basic cellular functions to response to environment. We have studied multicellular effect on chemotaxis in slime mold Dictyostelium discoideum. Migration behaviors during aggregation were analyzed quantitatively. It was found that cells harboring different Tgr proteins separate within a few minutes after contact, while cells harboring the same proteins migrated together. Cells surrounded by the same type moved faster and aligned polarities with others. Such coordinated behaviors were observed in only two cells isolated from aggregate. Taken together, self/non-self recognition via Tgr proteins modulates collective migration behavior. We also developed a microfluidics system to generate spatio-temporal chemoattractant field artificially. Using this system, we demonstrated cells were able to migrate directionally in such chemoattractant field.
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