2012 Fiscal Year Final Research Report
Analysis of the cell division patterning during plant early embryogenesis by developing the optical manipulation techniques
| Project/Area Number |
23870014
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphology/Structure
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 植物胚発生 / 光顕微操作 / 細胞分裂 |
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| Research Abstract |
Multicellular animals and plants develop from the single-celled zygote to form the mature embryo. During embryogenesis, the zygote follows a pattern of cell division to form the body plan of the embryo. In this study, I performed live-cell imaging using in vitroovule culture and the fluorescent marker lines in Arabidopsis thaliana. The data of live-cell imaging indicated that the timings of cell division are not strictly synchronized even in 4-cell embryo stage. Moreover, I established the gene induction system in a single-cell level in Arabidopsis embryo using the optical manipulation technique.
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[Journal Article] Programmed induction of endoreduplication by DNA double-strand breaks in Arabidopsis.2011
Author(s)
Adachi S., Minamisawa K., Okushima Y., Inagaki S., Yoshiyama K., Kondou Y., Kaminuma E., Kawashima M., Toyoda T., Matsui M., Kurihara D., Matsunaga S., Umeda M.
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 108
Pages: 10004-10009
DOI
Peer Reviewed
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