2012 Fiscal Year Final Research Report
he mechanisms of endocytic transport for activate receptor in budding yeast
| Project/Area Number |
23870027
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
TOSHIMA Junko 東京理科大学, 総合研究機構, ポストドクトラル研究員 (00431552)
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| Project Period (FY) |
2011 – 2012
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| Keywords | エンドサイトーシス / 膜輸送 |
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| Research Abstract |
To elucidate the mechanisms how activated receptor is recruited to clathrin coated pits, we screened yeast genome library, and identified Bmh2p, yeast homologue of 14-3-3 protein, as a binding protein for yeast GPCR. In addition, we tagged 13 human GPCR with GFP and succeeded in expressing in yeast cells. We also performed screening of about 80 % of yeast gene knock out strains for endocytosis defect, and identified about 200 genes that exhibited defects in endocytic transport.
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[Presentation] 出芽酵母Rab5ファミリータンパク質の細胞内輸送における役割, Arf GTPase活性化蛋白質Glo3pのエンドサイトー シス経路における役割2012
Author(s)
西ノ明祥, 十島純子, 古川大貴, 佐藤祥史, 十島二朗, 河田大樹, 富田剛史, 仲田瑛亮, 十島純子, 十島二朗
Organizer
第85回 日本生化学会大会
Place of Presentation
福岡
Year and Date
20121214-16
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