2012 Fiscal Year Final Research Report
Developmental study of the naso-hypophyseal placode in cyclostomes (Lamprey and Hagfish)
| Project/Area Number |
23870042
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Evolutionary biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SUGAHARA Fumiaki 独立行政法人理化学研究所, 形態進化研究グループ, 研究員 (00611005)
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| Project Period (FY) |
2011 – 2012
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| Keywords | ヤツメウナギ / ヌタウナギ / 鼻下垂体プラコード / 顔面形成 / 脊椎動物 |
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| Research Abstract |
In this study, we found that although lamprey naso-hypophyseal placode develops as a single median thickening, nasal- and hypophyseal placode specific genes were already expressed separately before placode thickening. In order to track the cell lineage of the nasal placodal area, we succeeded to inject DiI focally at the pre-placodal area. Also, to trace cell movements, we injected a fluorescence protein at 1 cell stage and succeeded to observe fluorescein signals in this animal. Regarding hagfishes, we obtained a few fertilized eggs. We are preparing to analyze their gene expression patterns.
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