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2012 Fiscal Year Final Research Report

Methodology development for acquisition of a numerous number of new natural products from filamentous

Research Project

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Project/Area Number 23880025
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionUniversity of Shizuoka

Principal Investigator

NAKAZAWA Takehito  静岡県立大学, 大学院・薬学研究科, 客員共同研究員 (80608141)

Project Period (FY) 2010 – 2012
Keywords糸状菌 / 酵母 / 生合成 / 異種発現
Research Abstract

A number of biosynthetic genes for fungal secondary metabolites were successfully reconstructed in yeast cells, and I successfully obtained natural products biosynthesized by them through heterologous production in yeast. I also developed molecular genetics systems for biosynthetic studies in some filamentous fungi. And I obtained a number of natural products, some of which are new/novel, through disruptions of genes involved in regulation of biosynthetic genes. I then found that several secondary metabolites are involved in regulation of transcription and sexual development (formation of sexual spores) in Chaetomium globosum.

  • Research Products

    (18 results)

All 2013 2012 2011 Other

All Journal Article (6 results) Presentation (11 results) Remarks (1 results)

  • [Journal Article] Cc.snf5, a gene encoding a putative component of the SWI/SNF chromatin remodeling complex, is essential for sexual development in the agaricomycete Coprinopsis cinerea.2013

    • Author(s)
      AndoY., T. Nakazawa, K. Oka, K. Nakahori, T. Kamada
    • Journal Title

      FungalGenet. Biol.

      Volume: 50 Pages: 82-89

    • DOI

      DOI:10.1016/j.fgb.2012.09.010

    • URL

      http://dx.doi.org/

  • [Journal Article] Combinatorial Generation of Complexity by Redox Enzymes in the Chaetoglobosin A Biosynthesis.2013

    • Author(s)
      K. Ishiuchi, T. Nakazawa, F. Yagishita, T. Mino, H. Noguchi, K. Hotta, K. Watanabe
    • Journal Title

      J. Am. Chem. Soc.

      Volume: accepted on Apr. 24th.

    • DOI

      DOI:10.1021/ja402828w

    • URL

      http://dx.doi.org/

  • [Journal Article] Establishing a new methodology for genome mining and biosynthesis of polyketides and peptides through yeast molecular genetics.2012

    • Author(s)
      K. Ishiuchi, T. Nakazawa, T. Ookuma, S. Sugimoto, M. Sato, Y. Tsunematsu, N. Ishikawa, H. Noguchi, K. Hotta, H. Moriya, K. Watanabe
    • Journal Title

      ChemBioChem

      Volume: 13 Pages: 846-854

    • DOI

      DOI:10.1002/cbic.201100798

  • [Journal Article] Overexpressing transcriptional regulator in Aspergillus oryzae activates a silent biosynthetic pathway to produce novel polyketide.2012

    • Author(s)
      T. Nakazawa, K. Ishiuchi, A. Praseuth, H. Noguchi, K. Hotta, K. Watanabe
    • Journal Title

      ChemBioChem

      Volume: 13 Pages: 855-861

    • DOI

      DOI:10.1002/cbic.201200107

  • [Journal Article] Overexpressing transcriptional regulator in Chaetomium globosum activates a silent biosynthetic pathway: Evaluation of shanorellin biosynthesis.2012

    • Author(s)
      Y. Tsunematsu, S. Ichinoseki, T. Nakazawa, N. Ishikawa, H. Noguchi, K. Hotta, K. Watanabe
    • Journal Title

      J. Antibiot

      Volume: 65 Pages: 377-380

    • DOI

      DOI:10.1038/ja.2012.34

    • URL

      http://dx.doi.org/

  • [Journal Article] 生合成遺伝子クラスターの高度強制発現による合成生物学が拓く有用天然物の創製2012

    • Author(s)
      中沢威人, 恒松雄太, 石川格靖, 渡辺賢二
    • Journal Title

      生物工学会誌

      Volume: 90 Pages: 289-292

    • URL

      https://www.sbj.or.jp/wp-content/.../9006_tokushu-1_3.pdf

  • [Presentation] 形態形成変異を指標とした、糸状菌Chaetomium globosum における休眠生合成遺伝子の活性化変異株のスクリーニング2013

    • Author(s)
      中沢威人、五反田康孝、石内勘一郎、野口博司、渡辺賢二
    • Organizer
      日本薬学会第133 年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      20130327-30
  • [Presentation] 形態形成変異を指標とした、糸状菌Chaetomium globosum における休眠生合成遺伝子の活性化変異株のスクリーニング2013

    • Author(s)
      中沢威人、五反田康孝、石内勘一郎、野口博司、渡辺賢二
    • Organizer
      日本農芸化学会2013 年度大会
    • Place of Presentation
      東北大学
    • Year and Date
      20130324-28
  • [Presentation] Molecular genetics studies on secondary metabolism in Chaetomium globosum reveal involvement of aureonitol and chaetoglobosins in gene regulation and sexual reproduction.2013

    • Author(s)
      T. Nakazawa, K. Ishiuchi, S. Sugimoto, Y. Gotanda, M. Sato, H. Noguchi, K. Watanabe
    • Organizer
      27th Fungal Genetics Conference
    • Place of Presentation
      Asilomar conference center, CA, USA
    • Year and Date
      20130312-17
  • [Presentation] 糸状菌Chaetomium globosum における天然物生合成の人為的変動2013

    • Author(s)
      中沢威人、五反田康孝、石内勘一郎、野口博司、渡辺賢二
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜バイオ大学
    • Year and Date
      20130308-10
  • [Presentation] Chaetomium globosumにおける天然物生合成遺伝子研究からみえてきた特定二次代謝産物による遺伝子発現制御および有性生殖への関与2012

    • Author(s)
      中沢威人、石内勘一郎、杉本覚、五反田康孝、佐藤道大、野口博司、渡辺賢二
    • Organizer
      第12回糸状菌分子生物学コンファレンス
    • Place of Presentation
      ウインクあいち
    • Year and Date
      20121112-13
  • [Presentation] 有用天然物の探索および生合成遺伝子クラスターの特定を目的とした糸状菌Chaetomium globosum veA遺伝子破壊株の作成2012

    • Author(s)
      中沢威人、石内勘一郎、五反田康孝、佐藤道大、杉本覚、野口博司、渡辺賢二
    • Organizer
      日本生薬学会第59回年会
    • Place of Presentation
      かずさアーク
    • Year and Date
      20120917-18
  • [Presentation] Biosynthesis of Natural Products Through a Fungal Molecular Genetics.2012

    • Author(s)
      T. Nakazawa, T. Ookuma, S. Sugimoto, S. Ichinoseki, Y. Gotanda, N. Ishikawa, Y. Tsunematsu, K. Ishiuchi, H. Noguchi, K. Watanabe
    • Organizer
      11th European Conference on Fungal Genetics
    • Place of Presentation
      Philipps-Universitat Marburg, Germany
    • Year and Date
      20120330-0402
  • [Presentation] 二次代謝研究を指向した糸状菌Chaetomium globosum の分子遺伝学実験システムの整備2012

    • Author(s)
      中沢威人、杉本覚、五反田康考、野口博司、渡辺賢二
    • Organizer
      日本農芸化学会2012 年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      20120322-26
  • [Presentation] Establishing a New Methodology for Genome Mining and Biosynthesis of Natural Products Through a Fungal Molecular Genomics.2011

    • Author(s)
      T. Nakazawa, K. Ishiuchi, K. Hotta, H. Noguchi, H. Moriya, K. Watanabe
    • Organizer
      8th AFMC International Medical Chemistry Symposium
    • Place of Presentation
      Keio Plaza Hotel Tokyo, Japan
    • Year and Date
      20111129-1202
  • [Presentation] A. oryzae からの不活性型二次代謝クラスター活性化と生合成産物の構造解析2011

    • Author(s)
      中沢威人、石内勘一郎、渡辺賢二
    • Organizer
      第11回糸状菌分子生物学コンファレンス
    • Place of Presentation
      東京大学弥生講堂
    • Year and Date
      20111116-17
  • [Presentation] Establishing a New Methodology for Genome Mining and Biosynthesis of Natural Products Through a Fungal Molecular Genomics.2011

    • Author(s)
      T. Nakazawa, K. Ishiuchi, K. Hotta, H. Noguchi, H. Moriya, K. Watanabe
    • Organizer
      4th International Conference on Health and LongevitySciences
    • Place of Presentation
      University of Shizuoka, Shizuoka, Japan
    • Year and Date
      2011-10-21
  • [Remarks]

    • URL

      http://www015.upp.so-net.ne.jp/kenji55-lab/

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Published: 2014-08-29  

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