2012 Fiscal Year Final Research Report
Dynamics of microtubules and plasma membrane domain during secondary cell wall deposition
| Project/Area Number |
23880029
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Wood science
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| Research Institution | Forestry and Forest Products Research Institute |
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Principal Investigator |
TAKATA Naoki 独立行政法人森林総合研究所, 森林バイオ研究センター, 研究員 (90605544)
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| Project Period (FY) |
2011 – 2012
|
| Keywords | セルロースミクロフィブリル / 細胞壁 / 表層微小管 / ポプラ |
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| Research Abstract |
Orientation of cellulose microfibril deposition is controlled by cortical microtubules. The aim of this study is to reveal dynamics of cortical microtubules during the secondary cell wall deposition. We generated transgenic aspens in which microtubules were labeled by GFP expression. Cortical microtubules were visualized by GFP fluorescence in leaf, root and phloem cells in the transgenic plants. However, GFP fluorescence was weak in xylem fiber cells due to low expression of GFP-tagged protein.
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