Analysis of intracellular granule that recognizes viral infection

2012 Fiscal Year Final Research Report

• Project/Area Number: 23890029
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Chiba University
• Principal Investigator: ONOMOTO Koji 千葉大学, 真菌医学研究センター, 助教 (10612202)
• Project Period (FY): 2011 – 2012
• Keywords: 自然免疫 / RLR / インターフェロン / ウイルス / RNA

Research Abstract

Emergence of new epidemic viruses such as swine flu and SARS spread rapidly and sometimes causes devastating impact. Innate immune system detects viral infection and protects host with viral sensors. Cytoplasmic viral RNA sensors, RLRs, sense viral RNA and initiate antiviral response, however it has been unclear how RLRs encounter viral RNA in cytoplasm. We revealed that RLRs localized in virus-induced granules together with viral RNA and trigger antiviral signal. We termed these aggregates as antiviral stress granules (avSGs).

Research Products

• [Journal Article] Functional Characterization of Domains of IPS-1 Using an Inducible Oligomerization System (2013)
  • Author(s): Takamatsu S, Onoguchi K, Onomoto K, Narita R, Takahasi K, Ishidate F, Fujiwara TK, Yoneyama M, Kato H, Fujita T
  • Journal Title: PLoS ONE Volume: 8(1) Pages: e53578
  • DOI: DOI:10.1371/journal.pone.0053578.
  • Peer Reviewed
• [Journal Article] Foreign RNA induces the degradation of mitochondrial antiviral signaling protein (MAVS): the role of intracellular antiviral factors (2012)
  • Author(s): Xing F, Matsumiya T, Onomoto K, Hayakari R, Imaizumi T, Yoshida H, Yoneyama M, Fujita T, Satoh K
  • Journal Title: PLoS ONE Volume: 7(9) Pages: e45136
  • DOI: DOI:10.1371/journal.pone.0045136.
  • Peer Reviewed
• [Journal Article] Critical role of an antiviral stress granule containing RIG-I and PKR in viral Detection and Innate Immunity (2012)
  • Author(s): Onomoto K, Jogi M, Yoo J, Narita R.Morimoto S, Takemura A, Sambhara S, Kawaguchi A, Osari S, Nagata K, Matsumiya T, Namiki H, Yoneyama M, Fujita T
  • Journal Title: PLoS ONE Volume: 7(8) Pages: e43031
  • DOI: 10.1371/journal.pone.0043031
  • Peer Reviewed
• [Journal Article] Identification of germicidal compound againstpicornavirus in bamboo pyroligneous acid (2012)
  • Author(s): Marumoto S, Yamamoto SP, Nishimura H, Onomoto K, Yatagai M, Yazaki K, Fujita T, Watanabe T
  • Journal Title: Journal of Agricultural and Food chemistry Volume: 60(36) Pages: 9106-9111
  • DOI: DOI:10.1021/jf3021317.
  • Peer Reviewed
• [Journal Article] Retinoic Acid-inducible Gene I-inducible miR-23b Inhibits Infections by Minor Group Rhinoviruses through Down- regulation of the Very Low Density Lipoprotein Receptor (2011)
  • Author(s): Ouda R, Onomoto K, Takahasi K, Edwards MR, Kato H, Yoneyama M, Fujita T
  • Journal Title: The Journal of Biological Chemistry Volume: 286(29) Pages: 26210-26219
  • DOI: DOI:10.1074/jbc.M111.229856
  • Peer Reviewed
• [Journal Article] Dysregulation of IFN system can lead to poor response to pegylated interferon and ribavirin therapy in chronic hepatitis C (2011)
  • Author(s): Onomoto K, Morimoto S, Kawaguchi T, Toyoda H, Tanaka M, Kuroda M, Uno K, Kumada T, Matsuda F, Shimotohno K, Fujita T, Murakami Y
  • Journal Title: PLoS ONE Volume: 6 (5) Pages: e19799
  • DOI: DOI:10.1371/journal.pone.0019799
  • Peer Reviewed
• [Book] 米山光俊ウイルスセンサーによるRNA認識とシグナル活性化の分子メカニズム実験医学増刊号「感染・共生・生体防御システム」
  • Author(s): 尾野本浩司
  • Total Pages: 3180-3187(8)
  • Publisher: 羊土社
• [Remarks] 葉大学真菌医学研究センター
  • URL: http://myco2.pf.chiba-u.jp/index.html
• [Remarks] 千葉大学真菌医学研究センタープロフィールページ
  • URL: http://myco2.pf.chiba-u.jp/profile/profile_onomoto.html
• [Remarks] 千葉大学真菌医学研究センター感染免疫分野ホームページ
  • URL: http://myco2.pf.chiba-u.jp/bunya_kansenmeneki/bunya_kansenmeneki/Mol._Immunol._jpn.html