2012 Fiscal Year Final Research Report
Development of diagnosis of intractable schizophrenia by the analysisof DNA methylation.
| Project/Area Number |
23890067
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical pharmacy
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| Research Institution | University of Toyama |
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Principal Investigator |
UNO Kyosuke 富山大学, 大学院・医学薬学研究部(薬学), 助教 (30608774)
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| Project Period (FY) |
2011 – 2012
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| Keywords | DNAメチル化 / 精神疾患 |
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| Research Abstract |
In this study, we focused DNA methylation as one of gene expression regulators. To analyze the DNA methylation variant, mice were treated with METH or saline for 6 days. Mice genomic DNA was extracted from NAc tissue and peripheral blood 2 h after the last injection. In METH treated mice, the methylation level of Shati/Nat8l promoter specific region prepared from NAc was lower than that of saline treated mice. Moreover, same domain of genome DNA extracted from cardiac blood in METH treated mice was less methylated compared with saline treated mice. These results indicate that expression of Shati/Nat8l might be regulated by DNA methylation, and to analyze the blood level of methylation at Shati/Nat8l specific genomic region could help a diagnosis of psychiatric disorders in clinical field.
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[Presentation] Involvement of Shati on depression-like behavior in the postpartum period in human and mice2012
Author(s)
Takaoka, K., Uno, K., Inagaki, R., Nagakura, M., Tamaji, A., Ozaki, N., Miyamoto, Y., Nitta, A.
Organizer
The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry / The 55th Annual Meeting of the Japanese Society for Neurochemistry
Place of Presentation
Kobe
Year and Date
20120929-1002
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[Presentation] The differences of the action of shati between the nucleus accumbens and dorsal striatum on the methamphetamine-indeuced addictive behaviors in mice2012
Author(s)
Sumi, K., Miyamoto, Y., Ishikawa, Y.,Iegaki, N., Muramatsu, S., Hibi, Y., Nabeshima, T., Uno, K., Nitta, A.
Organizer
The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry / The 55th Annual Meeting of the Japanese Society for Neurochemistry
Place of Presentation
Kobe
Year and Date
20120929-1002
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[Presentation] Overexpression of shati in the nucleus accumbens affects the abnormal behavior induced by methamphetamine in mice2012
Author(s)
Nitta, A., Ishikawa, Y., Iegaki, N., Muramatsu, S., Nabeshima, T., Furukawa-Hibi, Y., Uno, K., Miyamoto, Y.
Organizer
The 28^<th>CINP World Congress of Neuropsycchopharmacology
Place of Presentation
Stockholm, Sweden
Year and Date
20120603-07
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