2012 Fiscal Year Final Research Report
Analysis of extramedullary microenvironment includingcentral nerve system of leukemic cells
| Project/Area Number |
23890092
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Kyoto University |
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Principal Investigator |
KATO Itaru 京都大学, 医学研究科, 特定病院助教 (10610454)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 白血病 / 中枢神経再発 / 微小環境 |
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| Research Abstract |
Bone marrow samples from ALL patients were transplanted into NOG mouse and extramedullary organs including CNS showed the leukemic invasion. In the CNS, we observed the infiltration of leukemic cells that is consistent with pathology of human ALL patients. Several analyses suggest that our model recapitulates the CNS disease and histopathology of human ALL leukemic CNS. To identify genes involved in CNS leukemia development, we applied high-density oligonucleotide arrays to leukemic cells in CNS and BM. A series of CNS leukemic cell-specific gene sets have been successfully identified. Next, we transplanted leukemic cells harvested from CNS into NOG mice, and leukemic cells reconstitute the leukemia. Those results showed that leukemic cells in CNS contributed to the relapse of the disease with altering the gene expressionpattern. This information could be utilized for further mechanistic research and for devising optimized therapeutic strategies against CNS leukemia.
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Research Products
(7 results)
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[Journal Article] Clonal selection in xenografted TAM recapitulates the evolutionary process of myeloid leukemia in Down syndrome2013
Author(s)
Saida S, Watanabe KI, Sato-Otsubo A, Terui K, Yoshida K, Okuno Y, Toki T, Wang R, Shiraishi Y, Miyano S, Kato I, et al
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Journal Title
DOI
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