Anti-biofilm effects of azithromycin on Porphyromonas gingivalis

2012 Fiscal Year Final Research Report

• Project/Area Number: 23890103
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: MAEZONO Hazuki 大阪大学, 歯学部附属病院, 医員 (00613390)
• Project Period (FY): 2011 – 2012
• Keywords: バイオフィルム / 抗生物質 / ポルフィロモナス / ジンジバリス

Research Abstract

Anti-biofilm effects of azithromycin (AZM) on Porphyromonas gingivaliswere investigated using modified Robbins device. We examined protein expressions of AZM-treated/non-treated Porphyromonas gingivalis and found that protein expressions of sub-MIC AZM treated planktonic and biofilm-detached cells were different at 50 and 110 kDa. Proteins were turned out to be glutamate dehydrogenase, hemagglutinin protein and RagA protein.

Research Products

• [Journal Article] Porphyromonas gingivalis biofilms persist after chlorhexidine treatment (2013)
  • Author(s): Yamaguchi M, Noiri Y, Kuboniwa M, Yamamoto R, Asahi Y, Maezono H, Hayashi M, Ebisu S
  • Journal Title: Eur J Oral Sci Volume: Vol.121 Pages: 162-8
  • DOI: DOI:10.1111/eos.12050
  • Peer Reviewed
• [Journal Article] The sinR ortholog PGN_0088 encodes a transcriptional regulator that inhibits polysaccharide synthesis in Porphyromonas gingivalis ATCC 33277 biofilms (2013)
  • Author(s): Yamamoto R, Noiri Y, Yamaguchi M, Asahi Y, Maezono H, Kuboniwa M, Hayashi M, Ebisu S
  • Journal Title: PLoS One Volume: Vol.8, No.2 Pages: e56017
  • DOI: DOI:10.1371/journal.pone.0056017
  • Peer Reviewed
• [Journal Article] Antibiofilm effects of azithromycin and erythromycin on Porphyromonas gingivals (2011)
  • Author(s): Maezono H, Noiri Y, Asahi Y, Yamaguchi M, Yamamoto R, Izutani N, Azakami H, Ebisu S
  • Journal Title: Antimicrob Agents Chemother Volume: Vol.55, No.12 Pages: 5887-92
  • DOI: DOI:10.1128/AAC.05169-11
  • Peer Reviewed
• [Presentation] Azithromycin alters protein expressions in Porphyromonas gingivalisbiofilm-detached cells (2012)
  • Author(s): H. MAEZONO, Y. NOIRI, Y. ASAHI, M. YAMAGUCHI, R. YAMAMOTO, M. HAYASHI and S. EBISU
  • Organizer: 第60回国際歯科研究学会日本部会(JADR)
  • Place of Presentation: 新潟県新潟市
  • Year and Date: 2012-12-15
• [Presentation] Altered protein expressions by sub-minimum inhibitory concentrations of azithromycin on Porphyromonas gingivalis biofilms (2012)
  • Author(s): H. MAEZONO, Y. NOIRI, Y. ASAHI, M. YAMAGUCHI, R. YAMAMOTO, K. KUREMOTO, M. HAYASHI and S. EBISU
  • Organizer: ASM 6thconference on biofilms
  • Place of Presentation: Miami, USA
  • Year and Date: 2012-10-03
• [Presentation] Porphyromonas gingivalisバイオフィルムに対する各種抗菌薬の抗バイオフィルム効果 (2011)
  • Author(s): 前薗葉月、野杁由一郎、朝日陽子、山口幹代、山本れいこ、薮根敏晃、恵比須繁之
  • Organizer: 第134回日本歯科保存学会
  • Place of Presentation: 千葉県浦安市
  • Year and Date: 2011-06-10