2011 Fiscal Year Final Research Report
Establishment a model of human malignant mesothelial cells by down-regulation of microRNA-34
| Project/Area Number |
23890118
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Okayama University |
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Principal Investigator |
UENO Tsuyoshi 岡山大学, 岡山大学病院, 医員 (80509456)
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| Project Period (FY) |
2011
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| Keywords | microRNA / microRNA-34 / 中皮腫 / 正常中皮 |
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| Research Abstract |
Malignant mesothelioma(MM) is an aggressive tumor with a dismal prognosis. We previously reported that microRNA-34(miR-34) are methylated and down-regulated in MM and may play an important role in the carcinogenesis of MM. In this study, we down-regulated miR-34 in human mesothelial cells to investigate the cellular effect of miR-34 knockdown. miR-34 inhibitor transfection significantly increased cell proliferation. The invasive ability also increased in the miR-34 inhibitor transfectants. the up-regulation of c-Met, phospho-c-Met, and bcl-2 proteins were shown. In conclusion, the down-regulation of miR-34 induced an oncogenic phenotype in non-malignant mesothelial cells.
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