2012 Fiscal Year Final Research Report
Effects of IL-23 and Th17 on intestinal smooth muscle contractility and its underlying mechanism
| Project/Area Number |
23890149
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Kazuhiko 九州大学, 大学病院, 助教 (00274449)
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| Project Period (FY) |
2011 – 2012
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| Keywords | IL-23 / IL-17 / Th17 |
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| Research Abstract |
The objective of the present study was to investigate effects of IL-23 and IL-17, which are associated with Th17 immune response, on the intestinal smooth muscle. In the rat intestinal smooth muscle, tissue culture with IL-23 for 3 days slightly reduced contractile response to 118 mM K+, but not to carbachol. On the other hand, tissue culture with IL-17 significantly decreased the contractile responses both to 118 mM K+and carbachol. In α-toxin permeabilized smooth muscle strips, IL-17 did not change carbachol-induced Ca^2+-sensitization. These results indicated that Th17 immune response reduced contractility in the rat intestinal smooth muscle without changing Ca^2+-sensitization via a Ca^2+/calmodulin/MLCK pathway.
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