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2012 Fiscal Year Final Research Report

Development of in vivo hematopoietic differentiation system for human iPS cells

Research Project

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Project/Area Number 23890199
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionJichi Medical University

Principal Investigator

ABE Tomoyuki  自治医科大学, 医学部, 助教 (20610364)

Project Period (FY) 2011 – 2012
Keywords再生医学 / 幹細胞 / トランスレーショナルリサーチ / 移植・再生医療 / 応用動物
Research Abstract

We have shown the safety and effectiveness of treatment with busulfan in sheep and have suggested a conditioning regimen with busulfan for sheep in utero transplantation involving intravenous administration of 3 mg/kg busulfan to pregnant ewes 6 days befo

  • Research Products

    (18 results)

All 2013 2012 2011

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (14 results)

  • [Journal Article] Generation of Naive-like Porcine Induced Pluripotent Stem Cells Capable of Contributing to Embryonic and Fetal Development2013

    • Author(s)
      Fujishiro SH, Nakano K, Mizukami Y, Azami T, Arai Y, Matsunari H, Ishino R, Nishimura T, Watanabe M, Abe T, Furukawa Y, Umeyama K, Yamanaka S, Ema M, Nagashima H, Hanazono Y
    • Journal Title

      Stem Cells Dev

      Volume: 22(3) Pages: 473-482

    • DOI

      doi:10.1089/scd.2012.0173

    • Peer Reviewed
  • [Journal Article] Maternal administration of busulfan before in utero transplantation of human hematopoietic stem cells enhances engraftments in sheep2012

    • Author(s)
      Abe T, Masuda S, Tanaka Y, Nitta S, Kitano Y, Hayashi S, Hanazono Y, Nagao Y
    • Journal Title

      Exp Hematol

      Volume: 40(6) Pages: 436-444

    • DOI

      doi:10.1016/j.exphem.2012.01.018

    • Peer Reviewed
  • [Journal Article] Correspondence: Migration of cells from the yolk sac to hematopoietic tissues after in utero transplantation of early and mid gestation canine fetuses2011

    • Author(s)
      Masuda S, Hayashi S, Ageyama N, Shibata H, Abe T, Nagao Y, Hanazono Y
    • Journal Title

      Transplantation

      Volume: 92 Pages: e5-6

    • DOI

      doi:10.1097/TP.0b013e318222119f

    • Peer Reviewed
  • [Journal Article] Ex vivo expansion of human HSCs with Sendai virus vector expressing HoxB4 assessed by sheep in utero transplantation2011

    • Author(s)
      Abe T, Masuda S, Ban H, Hayashi S, Ueda Y, Inoue M, Hasegawa M, Nagao Y, Hanazono Y
    • Journal Title

      Exp Hematol

      Volume: 39(1) Pages: 47-54

    • DOI

      doi:10.1016/j.exphem.2010.09.007

    • Peer Reviewed
  • [Presentation] Generation of Naive-like Porcine Induced Pluripotent Stem Cells Capabe of Contributing to Embryonic and Fetal Development2012

    • Author(s)
      Shuh-hei Fujishiro, Kazuaki Nakano, Yoshihisa Mizukami, Takuya Azami, Yoshikazu Arai, Hitomi Matsunari, Takashi Nishimura, Masahito Watanabe, Tomoyuki Abe, Kazuhiro Umeyama, Shinya Yamanaka, Masatsugu Ema, Hiroshi Nagashima, and Yutaka Hanazono
    • Organizer
      MBSJ The 35th Annual Meeting
    • Place of Presentation
      Fukuoka
    • Year and Date
      20121211-14
  • [Presentation] ヒツジ胎子内におけるサルES 細胞の生着、造血系分化およびテラトーマ形成に及ぼす要因2012

    • Author(s)
      新田卓, 阿部朋行, 田中裕次郎, 佐々木京子, 増田茂夫, ボラジギン・サラントラガ, 林聡, 北野良博, 花園豊, 長尾慶和
    • Organizer
      第105回日本繁殖生物学会
    • Place of Presentation
      筑波
    • Year and Date
      20120905-08
  • [Presentation] Long-term Follow-up on the Engraftment of Human HSCs in Sheep after in Utero Transplantation; Society for Hematology and stem cells2012

    • Author(s)
      Yutaka Hanazono, Tomoyuki Abe, Suguru Nitta, Shigeo Masuda, Satoshi Hayashi, Yoshikazu Nagao
    • Organizer
      41st Annual Scientific Meeting
    • Place of Presentation
      Amsterdam, Netherland
    • Year and Date
      20120823-26
  • [Presentation] Possible factors for engraftment of monkey embryonic stem cells after in utero transplantation into sheep fetuses2012

    • Author(s)
      Yoshikazu Nagao, Tomoyuki Abe, Yujiro Tanaka, Kyoko Sasaki, Shigeo Masuda, Suguru Nitta, Borjigin Sarentonglaga, Satoshi Hayashi, Yoshihiro Kitano, Yutaka Hanazono
    • Organizer
      17th International Congress on Animal Reproduction
    • Place of Presentation
      Vancouver
    • Year and Date
      20120729-0802
  • [Presentation] HoxB4-Transduction of Human HSC Results in Longer-Term Engraftment Compared to Busulfan-Conditioning in Sheep after in Utero Transplantation2012

    • Author(s)
      Tomoyuki Abe, Suguru Nitta, Shigeo Masuda, Satoshi Hayashi, Yoshikazu Nagao and Yutaka Hanazono
    • Organizer
      The 18th Annual Meeting of Japan Society of Gene Therapy
    • Place of Presentation
      Kumamoto, Japan
    • Year and Date
      20120628-30
  • [Presentation] Long-Term Comparative Study on the Engraftment of Human Hematopoietic Stem Cells in Sheep2012

    • Author(s)
      Tomoyuki Abe, Suguru Nitta, Shigeo Masuda, Satoshi Hayashi, Yoshikazu Nagao and Yutaka Hanazono
    • Organizer
      The 10th Annual Meeting of International Society for Stem Cells Research
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      20120613-16
  • [Presentation] HoxB4-Transduction of Human HSC Results in Longer-Term Engraftment Compared to Busulfan-Conditioning in Sheep after in Utero Transplantation2012

    • Author(s)
      Tomoyuki Abe, Suguru Nitta, Shigeo Masuda, Satoshi Hayashi, Yoshikazu Nagao and Yutaka Hanazono
    • Organizer
      第10回 幹細胞シンポジウム
    • Place of Presentation
      淡路
    • Year and Date
      20120531-0602
  • [Presentation] ヒツジにおけるヒト造血細胞長期生着の条件検討2012

    • Author(s)
      阿部朋行、新田卓、増田茂夫、林聡、長尾慶和、花園豊
    • Organizer
      第59回 日本実験動物学会総会
    • Place of Presentation
      別府
    • Year and Date
      20120524-26
  • [Presentation] ブタを用いたiPS 細胞の免疫原性に関する検証2012

    • Author(s)
      水上喜久, 阿部朋行, 藤城修平, 柴田宏昭, 菱川修司, 小林英司, 花園 豊
    • Organizer
      第15回日本異種移植研究会
    • Place of Presentation
      京都
    • Year and Date
      2012-12-08
  • [Presentation] ヒツジにおけるヒト造血細胞の長期生着2012

    • Author(s)
      阿部朋行、増田茂夫、新田卓、林聡、長尾慶和、花園豊
    • Organizer
      第11回自治医科大学シンポジウム
    • Place of Presentation
      下野
    • Year and Date
      2012-08-31
  • [Presentation] 子宮内移植後のヒツジ体内におけるヒトおよびサル造血の長期生着に及ぼす要因2011

    • Author(s)
      新田卓, 阿部朋行, 増田茂夫, 林聡, 花園豊, 長尾慶和
    • Organizer
      第104回日本繁殖生物学会
    • Place of Presentation
      盛岡
    • Year and Date
      20110915-17
  • [Presentation] Conditioning With Busulfan Confers Engraftment Potential on Human Hematopoietic Stem Cell Comparable to HoxB4 Transduction in Sheep In Utero Transplantation2011

    • Author(s)
      Tomoyuki Abe, Shigeo Masuda, Nitta Suguru, Satoshi Hayashi, Yujiro Tanaka, Hiroshi Ban, Makoto Inoue, Mamoru Hasegawa, Yoshikazu Nagao, Yutaka Hanazono
    • Organizer
      The 9th Annual Meeting of International Society for Stem Cells Research
    • Place of Presentation
      Toronto, Ontario Canada
    • Year and Date
      20110615-18
  • [Presentation] ヒツジ胎子へのブスルファン投与はヒト造血幹細胞移植後の生着率を向上させる2011

    • Author(s)
      阿部朋行、増田茂夫、新田卓、林聡、花園豊、長尾慶和
    • Organizer
      第58回 日本実験動物学会総会
    • Place of Presentation
      東京
    • Year and Date
      20110525-27
  • [Presentation] ヒツジ胎子へのブスルファン前処置によるヒト造血幹細胞移植後の造血キメラ率の向上2011

    • Author(s)
      阿部朋行、増田茂夫、新田卓、林聡、長尾慶和、花園豊
    • Organizer
      第14回 日本異種移植研究会
    • Place of Presentation
      広島
    • Year and Date
      2011-12-10

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Published: 2014-08-29  

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