Tissue-selective heart failure treatment with the ischemic heart-homing peptide

2012 Fiscal Year Final Research Report

• Project/Area Number: 23890230
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Osaka Medical College
• Principal Investigator: KANKI Sachiko 大阪医科大学, 医学部, 助教 (40411350)
• Project Period (FY): 2011 – 2012
• Keywords: 心筋虚血 / アフィニティ精製 / ペプチド

Research Abstract

To find a receptor molecule of the ischemic heart-homing peptide, the affinity screening is necessary and the carrier must play a key role. FG beads, which are 200nm in diameter and consisted of magnetic ferrite covered by Poly-glycidylmethacrylate resin, are used as affinity carrier to bind the ischemic heart-homing peptides. The condition to bind the peptide to FG beads was optimized, and the combination of the acetylene peptide at N’-terminus and azide FG beads worked not destroy tertiary structure of peptide. Then, pull-down assay with the affinity beads was performed to find receptors for the homing peptide from ischemic heart tissue of rats, which is made by temporary occlusion of leftcoronary artery.