Molecular mechanisms of up-regulation of regulatory T cells induced by asbestos

2012 Fiscal Year Final Research Report

• Project/Area Number: 23890237
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Hygiene
• Research Institution: Kawasaki Medical School
• Principal Investigator: MATSUZAKI Hidenori 川崎医科大学, 医学部, 助教 (80335463)
• Project Period (FY): 2011 – 2012
• Keywords: アスベスト / がん / 腫瘍免疫 / 制御性 T 細胞 / アポトーシス

Research Abstract

We previously reported that MT-2 cells, model of regulatory T cells, showed resistance to high concentration of asbestos and up-regulation of IL-10 and TGFβ production after long-term exposure to low concentration of asbestos. In this study, we analyzed the involvement of transcription factors, FoxP3, NFAT and FoxO1, in change of functions of MT-2 cells induced by asbestos. It was revealed that long-term exposure to asbestos reduced expression of FoxP3 and FoxO1, and FoxO1 regulated apoptosis induced by asbestos. These results are important for elucidating the mechanism of up-regulation of regulatory T cell induced by asbestos.

Research Products

• [Journal Article] Alteration of cytoskeletal molecules in a human T cell line caused by continuous exposure to chrysotile asbestos. (2013)
  • Author(s): Maeda, M., Chen, Y., Kumagai-Takei, N., Hayashi, H., Matsuzaki, H., Lee, S., Hiratsuka, JI., Nishimura, Y., Kimura, Y., Otsuki T.
  • Journal Title: Immunobiology Volume: (in press)
  • DOI: DOI:10.1016/j.imbio.2013.04.007
  • Peer Reviewed
• [Journal Article] Effect of Asbestos Exposure on Differentiation of Cytotoxic T Lymphocytes in MLR of Human PBMCs. (2013)
  • Author(s): Kumagai-Takei, N., Nishimura, Y., Maeda, M., Hayashi, H., Matsuzaki, H., Lee, S., Hiratsuka, J., Otsuki, T.
  • Journal Title: Am. J. Respir. Cell Mol. Biol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Altered functions of alveolar macrophages and NK cells involved in asbestos-related diseases. (2013)
  • Author(s): Nishimura, Y., Maeda, M.,Kumagai-Takei, N., Lee, S., Matsuzaki, H., Wada, Y., Nishiike-Wada, T., Iguchi, H., Otsuki, T.
  • Journal Title: Environ. Health Prev. Med. Volume: 18(3) Pages: 198-204
  • DOI: DOI:10.1007/s12199-013-0333-y
  • Peer Reviewed
• [Journal Article] Resistance to asbestos-induced apoptosis with continuous exposure to crocidolite ona human T cell. (2012)
  • Author(s): Maeda, M., Yamamoto, S., Chen, Y.,Kumagai-Takei, N., Hayashi, H., Matsuzaki, H., Lee, S., Hatayama, T., Miyahara, N., Katoh, M., Hiratsuka, J., Nishimura, Y., Otsuki, T.
  • Journal Title: Sci. Total Environ. Volume: 429 Pages: 174-182
  • DOI: DOI:10.1016/j.scitotenv.2012.04.043.
  • Peer Reviewed
• [Journal Article] Asbestos-induced cellular and molecular alteration of immunocompetent cells and their relationship with chronic inflammation and carcinogenesis. (2012)
  • Author(s): Matsuzaki, H., Maeda, M., Lee, S., Nishimura, Y., Kumagai-Takei, N., Hayashi, H., Yamamoto, S., Hatayama, T., Kojima, Y., Tabata, R., Kishimoto, T., Hiratsuka, J., Otsuki, T.
  • Journal Title: J. Biomed. Biotechnol. Volume: 2012 Pages: 492608
  • DOI: DOI:10.1155/2012/492608.
  • Peer Reviewed
• [Journal Article] Environmental factors producing autoimmune dysregulation--chronic activation of T cells caused by silica exposure. (2012)
  • Author(s): Lee, S., Hayashi, H., Maeda, M., Chen, Y., Matsuzaki, H., Takei-Kumagai, N., Nishimura, Y., Fujimoto, W., Otsuki, T.
  • Journal Title: Immunobiology. Volume: 217(7) Pages: 43-48
  • DOI: DOI:10.1016/j.imbio.2011.12.009
  • Peer Reviewed
• [Journal Article] Functional and direct interaction between the RNA binding protein HuD and active Akt1. (2012)
  • Author(s): Fujiwara, T., Fukao, A., Sasano, Y., Matsuzaki, H., Kikkawa, U., Imataka, H., Inoue, K., Endo, S., Sonenberg, N., Thoma, C., Sakamoto, H.
  • Journal Title: Nucleic Acids Res Volume: 40(5) Pages: 1944-1953
  • DOI: DOI:10.1093/nar/gkr979
  • Peer Reviewed
• [Journal Article] Asbestos induces reduction of tumor immunity (2011)
  • Author(s): Kumagai-Takei, N., Maeda, M., Chen, Y., Matsuzaki, H., Lee, S., Nishimura, Y., Hiratsuka, J., Otsuki, T
  • Journal Title: Clin. Dev. Immunol Volume: 2011 Pages: 481439
  • DOI: DOI:10.1155/2011/481439
  • Peer Reviewed
• [Presentation] MT-2細胞のアポトーシス制御におけるFoxO1の役割 (2013)
  • Author(s): 松崎秀紀
  • Organizer: 第83回日本衛生学会学術総会
  • Place of Presentation: 金沢大学
  • Year and Date: 20130324-26
• [Presentation] フォークヘッド転写因子FoxO1を介したMT-2細胞のアポトーシス調節機構の解析 (2013)
  • Author(s): 松崎秀紀
  • Organizer: 第12回分子予防環境医学研究会
  • Place of Presentation: つくばサイエンス・インフォメーションセンター
  • Year and Date: 20130201-02
• [Presentation] The role of forkhead transcription factor FoxO1 in regulation of MT-2 cells (2012)
  • Author(s): Matsuzaki, H
  • Organizer: 第41回日本免疫学会学術総会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 20121205-07
• [Presentation] アスベスト長期曝露により誘導されるMT-2細胞の機能変化のメカニズムの解析 (2012)
  • Author(s): 松崎秀紀
  • Organizer: 第19回免疫毒性学会
  • Place of Presentation: 東京慈恵会医科大学
  • Year and Date: 20120915-16
• [Presentation] アスベスト曝露によるフォークヘッド転写因子FoxO1の機能調節 (2012)
  • Author(s): 松崎秀紀
  • Organizer: 第82回日本衛生学会学術総会
  • Place of Presentation: 京都大学
  • Year and Date: 20120324-26
• [Presentation] Effect of Asbestos onTranscription Factor Foxo1 (2012)
  • Author(s): Matsuzaki, H
  • Organizer: 30thinternational Congress onOccupational Health
  • Place of Presentation: Cancun Center(メキシコ)
  • Year and Date: 20120318-23
• [Presentation] Effect of asbestos onforkhead transcription factors FoxO1 in MT-2 Cells (2012)
  • Author(s): Matsuzaki, H
  • Organizer: 51st Annual Meeting of the Society of Toxicology
  • Place of Presentation: Moscone Center(米国)
  • Year and Date: 20120311-15
• [Presentation] THE ROLE OF TRANSCRIPTION FACTOR FOXO1 IN ASBESTOS-INDUCED APOPTOSIS OF MT-2CELLS (2012)
  • Author(s): Matsuzaki, H
  • Organizer: 52nd Annual Meeting of theSociety of Toxicology
  • Place of Presentation: Henry B. Gonzalez ConventionCenter(米国)
  • Year and Date: 20120311-14
• [Presentation] フォークヘッド転写因子FoxO1に対するアスベスト曝露の影響 (2011)
  • Author(s): 松崎秀紀
  • Organizer: 第18回日本免疫毒性学会学術大会
  • Place of Presentation: 千葉大学
  • Year and Date: 20110908-09
• [Remarks]
  • URL: http://www.kawasaki-m.ac.jp/hygiene/index.htm