2023 Fiscal Year Research-status Report
Structures and dynamics of ABC transporters MsbA and LptB2FG in working with LptC during transport activities
| Project/Area Number |
23K05713
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
NGO XUANKIEN 金沢大学, ナノ生命科学研究所, 特任助教 (60778190)
|
|---|
| Project Period (FY) |
2023-04-01 – 2026-03-31
|
| Keywords | ABC transporter / MsbA / LptC / HS-AFM / E. coli / FRET |
|---|
| Outline of Annual Research Achievements |
1)Successfully prepared 2D crystal structures of MsbA within lipid membranes containing both with and without Kdo2 lipid A; 2) Achieved high spatial and temporal imaging of both NBD and TMD sides of MsbA embedded in these lipid membranes; 3) Developed new methods for the automatic analysis of AFM data; 4) completed the construction of plasmids and transfected E. coli cells with the target plasmids aimed for FRET measurements.
|
| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
I am potentially investigating a novel pathway for the gate operation of Lipopolysaccharide (LPS) induced by ABC transporters MsbA and LptC in E. coli.
|
| Strategy for Future Research Activity |
In FY2024, my planned research agenda includes the following tasks: 1)Finalize the data analyses of AFM data; 2) Develop a protein-membrane model suitable for Molecular Dynamics simulation and/or Coarse-Grained simulation; 3) Investigate the interaction of MsbA with target protein in E. coli using FRET techniques; 4) Draft the manuscript detailing the findings.
|
| Remarks |
I am a co-chairman organizing The First International Symposium of Nano Life Science: NanoBioCoM2023.
https://www.icisequynhon.com/conferences/2023/nanobiocom2023/index.html
|
