2023 Fiscal Year Research-status Report
Expression regulation and function analysis of a novel immune checkpoint molecule ILDR2
| Project/Area Number |
23K09134
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
張 晨陽 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (40768363)
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| Project Period (FY) |
2023-04-01 – 2026-03-31
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| Keywords | ILDR2 / EAE |
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| Outline of Annual Research Achievements |
In this study, we investigated the expression profile of a novel B7 checkpoint molecule, ILDR2, and applied various mouse models to clarify the immunological functions of ILDR2 in cancer immunity and neuroimmunity. We aim to deepen our understanding of new immune tolerance induction mechanisms, explore the possibility of systemic immune regulation and develop novel immunotherapy for cancer and autoimmune diseases.By using of experimental autoimmune encephalomyelitis (EAE), a mouse model of MS, the applicant and colleagues has demonstrated that the development of Eomes+ Th cells, which are closely related to disease severity of chronic EAE and SPMS patients, are largely depended on disease associated microglia (DAM)
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
We have not yet evaluated the role of ILDR2 in EAE.
That is a key part to explore the function of ILDR2 in systemic immune regulation and to develop novel immunotherapy for cancer and autoimmune diseases.
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| Strategy for Future Research Activity |
We will examine whether administration of ILDR2-Fc into EAE mice could ameliorate disease progress. In addition, we will also evaluate whether disease progression will be accelerated by administration of the neutralizing antibody against ILDR2 into EAE mice. Moreover, the interaction between microglia or astrocyte and Th cells derived from CNS can be evaluated. To evaluate the antigen-specific inhibition of ILDR2, 2D2 TCR transgenic mice that express a myelin oligodendrocyte glycoprotein (MOG)-specific T cell receptor will be used.
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| Causes of Carryover |
To complete this study, we will use the fund for
1 chemical/antibody/plastics
2 test animals (mouse)
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