2023 Fiscal Year Research-status Report
Modeling plant adaptation in the context of climate change using genomics and epigenomics of stress tolerant Mangrove trees
| Project/Area Number |
23K11509
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
MIRYEGANEH Matin 沖縄科学技術大学院大学, 統合群集生態学ユニット, スタッフサイエンティスト (90793505)
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| Project Period (FY) |
2023-04-01 – 2026-03-31
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| Keywords | Mangroves / epigenetics / genomic / climate change |
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| Outline of Annual Research Achievements |
Since the beginning of my Kakenhi project (Apr. 2023) I have successfully extracted the DNA from 2 other mangrove species (Rhizopphora stylosa, Kandelia obovata) and sequenced the genome, using PacBio-Hifi long read sequencing. I then de-novo assembled and annotated the genome. As for Hi-C sequencing, the 3 species of mangroves (Bruguiera gymnorhiza, Rhizopphora stylosa, Kandelia obovata) are being processed and library preparation is under preparation. These annotated genomes are the prerequisite of the downstream epigenetic analyses.
In order to study the epigenetic stress memory, I have also done the DNA extraction and whole genome EM-seq sequencing for 96 samples (48 from Bruguiera gymnorhiza) and (48 from kandelia obovata). I have analyzed the data for the 48 samples from B. gymnorhiza and the results are very exciting. I also have extracted the RNA from the same samples and have sequenced them.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Everything is going as planned.
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| Strategy for Future Research Activity |
Next I will analyze the data to investigate the association between expression of the genes and methylated regions of DNA.
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| Causes of Carryover |
There was 110,183 yen left which was too small to use for sequencing so I transferred to this year to be used together with new budget for sequencing.
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Research Products
(1 results)
