2023 Fiscal Year Research-status Report
Single-cell Mitochondria Analysis using Molecularly Imprinted Material
| Project/Area Number |
23K13776
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| Research Institution | Kyushu University |
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Principal Investigator |
LIU CHENCHEN 九州大学, 理学研究院, 助教 (50963211)
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| Project Period (FY) |
2023-04-01 – 2025-03-31
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| Keywords | sample preconcentration / single cell analysis / electrophoresis / mass spectroscopy |
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| Outline of Annual Research Achievements |
This project aims to devise a technique for analyzing mitochondria at the single-cell level. This approach consists of a highly sensitive sample detection method and a novel material tailored for the collection of mitochondria. Currently, we have successfully developed a highly sensitive capillary electrophoresis method that offers 40 times improved detection compared to state-of-the-art technologies, specifically designed for profiling glycans. We intend to further develop and apply this analysis method for profiling cell samples.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
To profile the composition of mitochondria at the single-cell level, it is necessary to detect trace samples, collect mitochondria, and recover biomolecules. Overcoming the challenges related to specific organelle recognition and analyte preconcentration is crucial. We have made significant progress in achieving sensitive preconcentration and detection of standard glycan samples, and the study is advancing smoothly.
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| Strategy for Future Research Activity |
To achieve sensitive cellular analysis, our plan is to: (1) further investigate sensitive glycan detection and apply it to single-cell analysis, (2) develop new materials and technologies for the specific collection of cellular mitochondria, and (3) design a device platform for trace biomolecule detection. Furthermore, our objective is to develop a device for subcellular composition profiling in an integrated manner, aiming to prevent sample loss and ensure sensitivity. Ultimately, we aim to propose a proof-of-concept method for subcellular analysis as our final goal.
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| Causes of Carryover |
Due to the early stage of this project mainly relies on our already obtained materials, there are chances to adjust budget for more effective usage. Part of the budget is saved for the later stage of the project. The budget fee for next fiscal year would be used for factory fee of customized device, material fees for device fabrication, human labor fee for device test, and so on.
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