2023 Fiscal Year Research-status Report
Design and synthesis of shortwave-infrared (SWIR) fluorescent probes for specifically targeted molecular imaging of breast cancer.
| Project/Area Number |
23K13851
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2023-04-01 – 2026-03-31
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| Keywords | Fluorescence imaging / Organic synthesis / Breast cancer |
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| Outline of Annual Research Achievements |
The purpose of our research is to visualize deep living tissue through fluorescence imaging because fluorescence guided cancer surgery is very important for complete removal of solid tumors. So far visible and near-infrared (NIR) region as been used for clinical applications. For deep tissue visualization, shortwave-infrared (SWIR, >1000 nm) emitting fluorophores are important. I proposed to synthesize water soluble, biocompatible, and SWIR emitting organic fluorophores for deep tissue imaging of breast cancer. The intermediate compounds to synthesize proposed organic probe was unstable so, we alternatively developed organic probes which are highly efficient for the proposed biological applications.
We demonstrate that these probes are biocompatible and versatile, which can be conjugated to any antibodies and proteins. Using newly synthesized probes, we carried out in-vivo imaging of breast cancer. Also we demonstrate that these probes can be applicable for multicolor imaging of cancers and blood vessels. Currently, we are working on the development of clinically applicable probes with bright fluorescence for breast cancer imaging. The results obtained from this work is recently published in ACS Applied Materials and Interfaces 2024, 16, 14, 17253-17266.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Our aim was to develop biocompatible organic probes for deep tissue imaging of breast cancer. In the first year of the project period, we could synthesize biocompatible fluorescent probes and demonstrate in-vivo imaging of breast cancer. By using the information obtained so far we are proceeding further to develop more stable and clinically applicable probes for breast cancer imaging. The results obtained until now make this project proceed smoothly.
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| Strategy for Future Research Activity |
Some of the intermediate compounds needed to synthesize the proposed organic probe was very low so, we could synthesized alternate probes which are efficient for fluorescence imaging in mice experiments. The water solubility of the developed probes are relatively low, it limits these probes from the clinical application in human. In future we will be carrying out experiments to enhance their water solubility and perform cancer imaging animal model.
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| Causes of Carryover |
In the fiscal year 2023, the grant amount was mostly used for chemicals and research collaboration visit. In the fiscal year 2024, we are planning to carry out more animal experiments which required expensive protein and antibody molecules and a part of the grant amount will be spent for collaboration visit and to attend international conferences. the incurring amount will be properly used in the fiscal year 2024.
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