2023 Fiscal Year Research-status Report
Ion permeation and rotation mechanisms in bacterial flagellar motor stators
| Project/Area Number |
23K14154
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
TRAN PHUOC・DUY 東京工業大学, 生命理工学院, 助教 (50848546)
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| Project Period (FY) |
2023-04-01 – 2025-03-31
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| Keywords | Flagellar motor stators / MotA/B / Molecular dynamics |
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| Outline of Annual Research Achievements |
We have thoroughly making the well-equilibrated Flagellar motors MotA/B of the Bacillus subtilis, Campilobacter Jejuni and Clostridium sporogenes by performing the multiple trials, long molecular dynamics simulation (MD). The MotA/B proteins are embeded on the lipid bilayer membrane alike to the bacteria membrane. After that, we have performed detailed analysis of the ions interacting to the MotA/B proteins. After that, we have used enhanced sampling technique to drive the ions moving from periplasm to cytoplasm. The results help us to distinguish key interaction residues on MotA/B proteins that help ions to go through periplasm to cytoplasm without being trapped.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
We have identified the ionic penetrating mechanism of the Bacillus subtilis MotA/B successfully and currently extend to complete for the other species.
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| Strategy for Future Research Activity |
In the future, we will remodel the full length of the MotB protein and identify the mechanism of the plug region as well as periplasmic domain of MotB contributing to the selectivity, ion penetrating or non-ion penetrating.
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