2023 Fiscal Year Research-status Report
Evolutionary forces driving the diversity of protist symbioses
| Project/Area Number |
23K14256
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
HUSNIK Filip 沖縄科学技術大学院大学, 進化・細胞・共生の生物学ユニット, 准教授 (30886130)
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| Project Period (FY) |
2023-04-01 – 2025-03-31
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| Keywords | symbiosis / protist / bacteria / archaea / genomics / organelle / evolution / mitochondrion |
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| Outline of Annual Research Achievements |
Our major published achievements so far include work on 7 (6 symbiont-derived) genomes in a single cryptomonad cell (George et al., Cur Biol 2023) and a study showing that previously reported symbionts in Symbiomonas were misidentified giant viruses (Cho et al., PLoS Genetics, 2024).
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
Due to the solid preparation status with many samples already collected or cultured in my group, the initial plan to screen a diversity of protists for symbionts is largely finished.
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| Strategy for Future Research Activity |
In the FY2024, we will proceed with a detailed characterization of the selected systems. So far, we published our work on 2 species (Cryptomonas and Symbiomonas). A manuscript on 7 species of large benthic foraminifera is currently drafted and additional manuscripts on >5 systems are in preparation. We will thus reach the number of ~10 characterized systems as proposed in the application.
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| Causes of Carryover |
Major costs of this project relate to sequencing where we have to fill a sequencing lane with our samples and the numbers are difficult to plan since sequencing coverage of protists is highly variable depending on genome size. The incurring amount will be used in the next fiscal year for laboratory consumables required for the detailed symbiont characterization.
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[Journal Article] A single cryptomonad cell harbors a complex community of organelles, bacteria, a phage, and selfish elements2023
Author(s)
George EE, Barcyte D, Lax G, Livingston S, Tashyreva D, Husnik F, Lukes J, Elias M, Keeling PJ
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Journal Title
Current Biology
Volume: 33
Pages: 1982-1996
DOI
Peer Reviewed / Int'l Joint Research
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