2023 Fiscal Year Research-status Report
Study of clinically over-expressed and chimeric RND multidrug efflux pumps from Acinetobacter baumannii and Pseudomonas aeruginosa
| Project/Area Number |
23K14346
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| Research Institution | Osaka University |
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Principal Investigator |
ZWAMA MARTIJN 大阪大学, 産業科学研究所, 特任准教授(常勤) (40827052)
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| Project Period (FY) |
2023-04-01 – 2025-03-31
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| Keywords | RND / Transporter / Multidrug Resistance / MDR / Efflux Pump |
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| Outline of Annual Research Achievements |
One of the leading causes of clinical multidrug-resistant (MDR) bacterial hospital isolates is the over-expression of multidrug efflux pumps belonging to the Resistance-Nodulation-cell Division (RND) protein superfamily. We aimed to understand the roles of efflux pumps in clinical isolates to MDR and also study less-studied clinically relevant pumps. During the first fiscal year of this research project, we started the first cloning of RND-type efflux pumps for this project and created active pumps. The results show interesting, novel findings that are relevant and important to the field of multidrug resistance. The preliminary results were very interesting, and we are currently continuing to investigate the phenotypes to help us understand multidrug resistance caused by the RND-type efflux pumps of P. aeruginosa, E. coli, and A. baumannii. Gene expression analysis was also performed, which gave us detailed information about the resistance factors in the resistant bacteria. In this fiscal year, we were also able to publish three papers, including drafting multiple manuscripts and laying the groundwork for additional research and publications in the near future, likely for the next fiscal year.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
The research went smoothly because there were no problems with cloning, and experimental results showed interesting preliminary results already.
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| Strategy for Future Research Activity |
Continue with the research as planned and perform experiments based on the positive procedures and results.
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| Causes of Carryover |
Because the cloning and experiments were going very smoothly, we did not need as much Article Costs, which we could spend on other things very useful for the study. Most of the grant was used, about about 20% is carried over to the next fiscal year where the budget is also a lot less, so it will be very usefull.
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