2023 Fiscal Year Research-status Report
The development of novel pulp capping material utilizing colloidal platinum nanoparticles and polymer-based components
| Project/Area Number |
23K16013
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| Research Institution | Hokkaido University |
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Principal Investigator |
劉 雲青 北海道大学, 歯学研究院, 博士研究員 (50962320)
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| Project Period (FY) |
2023-04-01 – 2026-03-31
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| Keywords | direct pulp capping / calcium hydroxide / platinum nanoparticles / angiogenesis / inflammatory |
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| Outline of Annual Research Achievements |
We aim to develop an experimental pulp capping material by the incorporation Colloidal platinum nanoparticles (CPN) with the commercially available pulp capping agent calcium hydroxide (CH). Initially, we assessed the pulpal tissue response in Wistar rats following the application of three different CPN materials at day 1, 7, 14, and 28 subsequent to direct pulp capping. The dental pulps were mechanically exposed and randomly divided into three groups based on the CPN materials applied: group 1- PAA-PT (platinum, poly acrylic acid, water), group 2- C-PT (platinum, sodium citric acid, water) and group 3- C-CYD (platinum, sodium citric acid, γ-cyclodextrin, water). All cavities were then restored using Super Bond-SB. A negative control (SB) was also included. At day 1, no or mild inflammatory responses were observed in all three CPN groups, significantly differing from the SB group (p<0.05), except C-PT group. At day 3, numerous blood vessels with no or mild inflammation were observed in the C- CYD group, indicating rapid angiogenesis, which was significantly different from SB groups (p<0.05), whereas PAA-PT and C-PT groups showed mild to moderate inflammatory responses. At day 7, no or mild inflammation was observed in the C-CYD group significantly differing from the PAA-PT, C-PT and SB groups (p<0.05), whereas mild or moderate inflammatory responses were observed in all other groups.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Last year, we evaluated the healing responses of the colloidal platinum nanoparticles (CPN).
CPN is being incorporated into pulp capping agent calcium hydroxide (CH). A positive control (CH) is also included.
The expression of angiogenesis, inflammation, and mineralization-related markers such as CD34, CD45, VEGFs; IL-6, IL-10; ALP, Runx2, OCN (immunohistochemistry) will be checked.
The mechanical properties including microhardness, compressive strength, setting time will be checked for screening out the optimal experimental pulp capping material.
On the other hand, the adhesion ability of the experimental pulp capping material to dental hard tissue is being evaluated (SEM, TEM, EDS, and FTIR).
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| Strategy for Future Research Activity |
Histological preparation: by the end of October 2024, the histological response of exposed dental pulp to the combined material will be evaluated using Hematoxylin-Eosin staining (histological observation), details were taken concerning the hard tissue bridge (continuity, morphological aspects, thickness, localization); Mechanical properties analysis: by the end of December 2024; Immunohistochemical preparation: by the end of August 2025; Data acquisition: by the end of September 2025; Data analysis: by the end of October 2025; Preparation of a draft for the manuscript: by the end of January 2026; Manuscript submission for publication: by the end of March 2026.
In our present study evaluation, the healing responses of colloidal platinum nanoparticles on dental pulp were very satisfactory. Therefore, calcium hydroxide combined with colloidal platinum nanoparticles could be the material of choice for direct pulp capping, which will be our next future research work.
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| Causes of Carryover |
The total budget for purchase of rats is the center of the research. The rats will be used for operation and then tissue extraction. For checking the quality of the experimental material regarding the adhesion ability, SEM/TEM technique are necessary. Diamond knives are already equipped in our lab. Research consumables include equipments, device and materials.
Histological and immunohistological staining are necessary to observe the pulpal response. Research consumables include reagents (Isoflurane, Vetorphale, Domitor, Dormicum, paraformal dehyde, EDTA, BSA, DAB, H2O2) and antibodies (CD34, CD45, VEGFs; IL-6, IL-10; ALP, Runx2, OCN).
As international travel expenses, I've recorded IADR and IAD. They’re very important international meet ings, which would provide me with the opportunity of discussing my results with renowned foreign researc hers. It might also help me to have favorable peer review during article submission.
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