Development of a novel reprogramming method incorporating extracellular pressure environment

2024 Fiscal Year Final Research Report

• Project/Area Number: 23K16178
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Institute of Science Tokyo (2024); Tokyo Medical and Dental University (2023)
• Principal Investigator: Sugimoto Asuna 東京科学大学, 東京科学大学病院, 助教 (80823830)
• Project Period (FY): 2023-04-01 – 2025-03-31
• Keywords: PIEZO1 / メカノセンシティブイオンチャネル / メカニカルストレス / 間葉系幹細胞 / 線維芽細胞

Outline of Final Research Achievements

In this study, to analyze the downstream signaling mechanisms of PIEZO1, we investigated the effects of extracellular pressure on bone marrow-derived mesenchymal stem cells. Focusing on PIEZO1-mediated calcium ion influx and ERK phosphorylation, we found that the influx of extracellular calcium is critical for ERK activation upon PIEZO1 activation. Furthermore, it was suggested that the R-Ras-binding domain of PIEZO1 is involved in this signaling pathway.

Free Research Field

小児歯科

Academic Significance and Societal Importance of the Research Achievements

細胞外圧負荷による細胞の分化誘導技術とダイレクトリプログラミングの技術を融合させることで，新たな分化誘導法が開発できる。本研究での研究成果は、PIEZO1の分子機構を応用した分化誘導法の開発の基盤となりうる。