2023 Fiscal Year Research-status Report
Real-time and high-throughput observation of nanomedicine behaviors of inside cancer cell spheroids with an integrated model of light sheet fluorescence microscopy and a microfluidic platform
| Project/Area Number |
23K19100
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| Research Institution | Chuo University |
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Principal Investigator |
余 雪萍 中央大学, 理工学研究所, 専任研究員 (80980143)
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| Project Period (FY) |
2023-08-31 – 2025-03-31
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| Keywords | Spheroids / Spheroids focusing / High throughput |
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| Outline of Annual Research Achievements |
Firstly, 2 types of 3D cancer cell spheroids with round shape, high uniformity and cell viability, and long-time cultivability were successfully generated by bio-printer. And the size of the 3D cancer spheroids can be easily controlled by adjusting cell concentrations and bio-printer settings (needle size, extrusion pressures, and extrusion time).
Secondly, a 3D cancer cell spheroids focusing system was successfully constructed. The 3D cancer cell spheroids were flowed by a lamina flow and observed with two cameras in different directions to obtain the length and width of spheroids to provide the 3D shape informtion.
Finally, a self-made 3D shape analysis software was designed. The video taken during the spheroids flowing can be upload to the software, which can analyze the shape information of each spheroid automatically.
Therefore, the generation method of spheroids with different sizes and high throughput analysis system have been confirmed. The different size of spheroids will be used to evaluate the nano-medicine uptake profile. The high throughput analysis system will be applied to realize the high-throughput observation, which is planned as the goal of 2024-2025 in the proposed research.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The research schedule was slightly changed. The spheroids focusing study (goal of 2024-2025) was firstly carried out.
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| Strategy for Future Research Activity |
Firstly, the light-sheet fluorescence microscopy will be constructed through using a cylinder lens to observe the inside of 3D cancer cell spheroids.
Secondly, the constructed light-sheet fluorescence microscopy will be integrated with the spheroids focusing system. Large amounts of 3D cancer cell spheroids will be focused by the spheroids focusing system. At the same time, the light-sheet fluorescence microscopy will capture the comprehensive information inside the 3D structure.
Finally, the 3D cancer cell spheroids will be treated with nano-medicine and observed with the constructed system to obtain the comprehensive dynamic behaviors of nanomedicine inside the 3D cancer cell spheroids.
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| Causes of Carryover |
Lots of equipment study has been done with the equipments in Hayakawa lab. The left money of 2023 will be used to buy materials for the nanomedicine analysis in 2024.
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