2023 Fiscal Year Research-status Report
pH‐responsive micelles with lower immunogenicity for smart drug delivery
| Project/Area Number |
23K19230
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| Research Institution | Kawasaki Institute of Industrial Promotion Innovation Center of NanoMedicine |
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Principal Investigator |
Mohamed Aziz・Awaad・Aziz 公益財団法人川崎市産業振興財団(ナノ医療イノベーションセンター), ナノ医療イノベーションセンター, 研究員 (50978286)
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| Project Period (FY) |
2023-08-31 – 2025-03-31
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| Keywords | polycarboxybetaine / immunogenicity / anti-tumor / drug delivery systems |
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| Outline of Annual Research Achievements |
Successfully were prepared a polyzwitterion termed as PGLu(DET-car) was prepared with a pH-responsive against tumorous pH and with lower immunogenicity compared with conventional polymers. The PGLu(DET-car) polymer showed lower induction of serum immunoglobulin (IgM, IgG) in vivo and cytokines (IL-2, IFN‐γ) induction in vitro compared with polyethylene glycol (PEG) polymer. Additionally, conjugation of PGLu(DET-car) into PEG polymer reduced the Anti-PEG IgM in the blood serum. The polymeric micelles prepared from the PGLu(DET-car) showed compatible affinity to reach deeper in the tumor site compared with those prepared from PEG. Furthermore, the PGLu(DET-car) micelles showed lower induction of IgM in the blood serum, as well as lower induction of IL-2, IFN‐γ cytokines in vitro.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
We successfully prepared a pH-responsive PGLu(DET-car)-based micelle. Additionally, we evaluated the immunogenicity of the prepared micelle both in vitro and in vivo. The current data showed lower immunogenicity of PGLu(DET-car) polymer as well as PGLu(DET-car)-based micelle compared with conventional polymers.
Unfortunately, we found difficulty loading a drug in the prepared micelles. The drug-loaded micelle preparation still needs more characterization and evaluation. we found unstable micelle after loading with anti-cancer drugs. therefore, it will take some time to optimize he conditions of loading drugs in the prepared micelles.
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| Strategy for Future Research Activity |
During the current year we will evaluate the in vivo biodistribution of PGLu(DET-car)-based micelle. Additionally, anti-cancer or anti-aging drugs loading into the prepared micelle will be optimized and evaluated using different animal models.
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| Causes of Carryover |
In addition to fund for the next fiscal year, we will use the remaining fund from previous year to buy further animal models, antibodies as well as some publication or patent fees.
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[Presentation] Changeable Net Charge on Gold Nanoparticles Facilitates Intratumor Accumulation, Retention and Penetration2023
Author(s)
Aziz Awaad, Hiroyasu Takemoto, Muneaki Iizuka, Koichi Ogi, Yuki Mochida, Abdul-Hackam Ranneh, Masahiro Toyoda, Makoto Matsui, Takahiro Nomoto, Kotaro Hayashi, Keishiro Tomoda, Tomoyuki Ohtake, Yutaka Miura, and Nobuhiro Nishiyama
Organizer
Third International Conference of the Faculty of Pharmacy, Ain Shams University (ICPASU 2023)
